斑蝥素酸镁对人肝癌细胞SMMC-7721裸鼠皮下移植瘤凋亡的影响

doi:10.16333/j.1001-6880.2015.02.008

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (2): 236-240.doi: 10.16333/j.1001-6880.2015.02.008

斑蝥素酸镁对人肝癌细胞SMMC-7721裸鼠皮下移植瘤凋亡的影响

晏容1，刘云2，朱欣婷1，易小飞1，刘流1，张云辉3，李晓飞1*

1 遵义医学院基础医学院；2 遵义医学院医学与生物学研究中心，遵义563003；3 贵州柏强制药有限公司，贵阳 550001

出版日期:2015-02-15 发布日期:2015-02-15

Effect of Magnesium Cantharidate on the Apoptosis of Subcutaneous Transplantation Tumor from Human Hepatoma Cells SMMC-7721 in Nude Mice

YAN Rong1, LIU Yun2, ZHU Xin-ting1, YI Xiao-fei1, LIU Liu1, ZHANG Yun-hui3, LI Xiao-fei1*

1 Basic Medicine School,Zunyi Medical College;2 Medical and Biological Research Center,Zunyi Medical College, Guizhou Zunyi 563003,China;3 Guizhou Baiqiang Pharmaceutical,Guizhou Guiyang 550001,China

Online:2015-02-15 Published:2015-02-15

摘要/Abstract

摘要： 建立人肝癌细胞SMMC-7721裸鼠皮下移植瘤模型，实验组每只裸鼠瘤周注射斑蝥素酸镁6.26×10_-5 mmol，对照组给予相同容积的无菌生理盐水瘤周注射。给药22 d后，观察斑蝥素酸镁对皮下移植瘤增殖的影响，并在此基础上，利用HE染色观察药物对人肝癌裸鼠皮下移植瘤组织形态学特征的影响。实验发现斑蝥素酸镁组移植瘤细胞体积变小、胞浆固缩、嗜酸性变，细胞核固缩、碎裂。透射电镜观察人肝癌裸鼠皮下移植瘤组织超微结构的改变，镜下见斑蝥素酸镁组移植瘤细胞核膜基本消失、核染色质聚集成团等改变。免疫组化二步法检测人肝癌裸鼠皮下移植瘤组织中bcl-2、bax的表达水平，结果显示斑蝥素酸镁组瘤组织中bcl-2的表达低于生理盐水组，而bax的表达高于生理盐水组（P<0.05）。本实验提示斑蝥素酸镁能明显抑制人肝癌裸鼠皮下移植瘤的增殖，并能诱导移植瘤细胞发生凋亡，其机制可能与上调bax和下调bcl-2表达有关。

关键词: 斑蝥素酸镁, 裸鼠, 皮下移植瘤, 细胞凋亡

Abstract: The model of subcutaneous transplantation tumor from human hepatoma cells SMMC-7721 in nude mice was established.Each nude mice was injected with magnesium cantharidate (6.26×10^-5mmol) around the tumor in experimental group,while the same volume of sterile saline was injected as control group.After administration for 22 days,the effect of magnesium cantharidate on the proliferation of subcutaneous transplantation tumor was observed.On the basis of that,the histomorphologic characteristics of subcutaneous transplantation tumor in nude mice was observed by HE staining.HE staining showed that the volume of transplantation tumor cells in magnesium cantharidate group became smaller,cytoplasm pyknosis,acidophilic degeneration,nuclear pyknosis and fragmentation.The ultra-structural changes of subcutaneous transplantation tumor in nude mice was observed by transmission electron microscopy.The results showed that the nuclear membrane of transplantation tumor in magnesium cantharidate group disappeared,chromatin gathered together,etc.The expression level of bcl-2 and bax was detected by Two-step immunohistochemistry.The results showed that the expression of bcl-2 in magnesium cantharidate group was obviously lower,but the expression of bax was higher than that of control group (P<0.05).The study indicated that magnesium cantharidate can inhibit the proliferation of subcutaneous transplantation tumor from human hepatoma cells in nude mice and induce the apoptosis of transplantation tumor cells.The mechanism may be related with the up-regulation of the expression of bax and down-regulation of the expression of bcl-2.

Key words: magnesium cantharidate, nude mice, subcutaneous transplantation tumor, cell apoptosis

中图分类号:

R93

晏容1，刘云2，朱欣婷1，易小飞1，刘流1，张云辉3，李晓飞1*. 斑蝥素酸镁对人肝癌细胞SMMC-7721裸鼠皮下移植瘤凋亡的影响[J]. 天然产物研究与开发, 2015, 27(2): 236-240.

YAN Rong1, LIU Yun2, ZHU Xin-ting1, YI Xiao-fei1, LIU Liu1, ZHANG Yun-hui3, LI Xiao-fei1*. Effect of Magnesium Cantharidate on the Apoptosis of Subcutaneous Transplantation Tumor from Human Hepatoma Cells SMMC-7721 in Nude Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(2): 236-240.

[1]	方雨潇, 王淑美. 山柰酚通过线粒体凋亡通路诱导三阴性乳腺癌细胞凋亡的机制研究[J]. 天然产物研究与开发, 2023, 35(3): 397-404.
[2]	温馨, 胡锦航, 程敏, 宋忠兴. 基于网络药理学、分子对接技术及体外实验验证探讨龙葵治疗乳腺癌的作用机制[J]. 天然产物研究与开发, 2023, 35(10): 1782-1793.
[3]	王姣姣, 袁平川, 许寒池, 陈颖, 张斯佳, 田梦云, 余子琪, 柳春燕. 禾谷镰刀菌胞外多糖诱导SGC-7901细胞凋亡作用及其机制研究[J]. 天然产物研究与开发, 2022, 34(7): 1204-1212.
[4]	孙婷婷, 刘洋, 李卓柯, 宗时宇, 支文冰, 狄志彪, 李晔, 张红. 黄精凝集素PCL-2诱导人前列腺癌LNCap细胞凋亡及机制研究[J]. 天然产物研究与开发, 2022, 34(2): 255-262.
[5]	刘娟汝, 彭成, 何育霖, 彭芳, 蒙春旺, 熊亮. 茺蔚子中木脂素类化学成分及肝细胞保护作用研究[J]. 天然产物研究与开发, 2022, 34(10): 1699-1706.
[6]	邓佳丽, 李晓, 安玉艳, 林泽崑, 汪良驹. 无花果叶片多糖抑制胃癌细胞增殖与促进凋亡效应[J]. 天然产物研究与开发, 2021, 33(8): 1282-1291.
[7]	孟令帅, 曹森, 彭琳, 马立志, 孟宪军, 周笑犁. 黑果腺肋花楸花色苷通过Nrf2机制对Aβ_1-42诱导SH-SY5Y细胞产生氧化应激及细胞凋亡的保护作用[J]. 天然产物研究与开发, 2021, 33(7): 1086-1095.
[8]	李晓明, 荣华, 潘思文, 董妙先. 天麻素联合异钩藤碱通过线粒体途径抑制MPP⁺诱导PC12细胞凋亡[J]. 天然产物研究与开发, 2021, 33(7): 1096-1101.
[9]	高鸣乡#, 蒋佩文#, 李敏惠, 杨平, 代敏. 草果挥发油诱导鼻咽癌6-10B细胞凋亡的机制研究[J]. 天然产物研究与开发, 2021, 33(6): 992-997.
[10]	李佩佩, 郝钰, 杨磊. 虎杖苷通过激活TrkA抑制芬太尼诱导的海马神经元细胞凋亡[J]. 天然产物研究与开发, 2021, 33(4): 630-637.
[11]	朱洪竹, 朱梅菊. 有氧运动联合螺旋藻营养补充对2型糖尿病大鼠学习记忆的影响及其机制[J]. 天然产物研究与开发, 2021, 33(11): 1845-1853.
[12]	刘国安, 靳亚东, 冉苗苗, 李贵琛, 杨麒励, 丁兰 . 姜黄素通过促进ROS产生诱导人脑胶质瘤细胞凋亡[J]. 天然产物研究与开发, 2020, 32(4): 541-548.
[13]	李卫强1,2*，王骄1，关芳1，艾梦环1. 宁夏密点麻蜥不同部位含药大鼠血清对人胃癌细胞凋亡的影响研究[J]. 天然产物研究与开发, 2019, 31(8): 1447-1451.
[14]	覃月穆1,2，姜泽群1,2，马艳霞2，张旭1,2*. 异甘草素对人肺癌细胞株 H460 增殖、凋亡及NF-κB信号通路的影响[J]. 天然产物研究与开发, 2018, 30(5): 847-855.
[15]	张雯珺1，屠凌岚1，吴丽梅1，高海峰2，黄萍萍2，郑晓亮1，王孝举1*. 橘络活性成分小分子果胶治疗非小细胞肺癌作用及机制研究[J]. 天然产物研究与开发, 2018, 30(4): 575-581.

斑蝥素酸镁对人肝癌细胞SMMC-7721裸鼠皮下移植瘤凋亡的影响

Effect of Magnesium Cantharidate on the Apoptosis of Subcutaneous Transplantation Tumor from Human Hepatoma Cells SMMC-7721 in Nude Mice

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价