肉苁蓉中松果菊苷和毛蕊花糖苷的植物雌激素活性研究

doi:10.16333/j.1001-6880.2015.03.002

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (3): 377-380.doi: 10.16333/j.1001-6880.2015.03.002

肉苁蓉中松果菊苷和毛蕊花糖苷的植物雌激素活性研究

王琳琳，李薇，宋新波*，余河水，苗琳，张丽娟，陶蕊

天津中医药大学，天津 300193

出版日期:2015-03-30 发布日期:2015-04-07

Study on Estrogenic Effects of Echinacoside and Actsoside from Herba Cistanche

WANG Lin-lin, LI Wei, SONG Xin-bo*, YU He-shui, MIAO Lin, ZHANG Li-juan, TAO Rui

Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China

Online:2015-03-30 Published:2015-04-07

摘要/Abstract

摘要： 研究肉苁蓉中松果菊苷和毛蕊花糖苷的雌激素活性。采用荧光素酶报告质粒法检测受试药物松果菊苷和毛蕊花糖苷的雌激素活性；利用雌激素受体竞争性结合实验和荧光素酶报告质粒法，检测松果菊苷和毛蕊花糖苷与雌激素的竞争性作用。松果菊苷和毛蕊花糖苷有上调雌激素应答元件（ERE）活性，和雌激素共同作用可以拮抗雌激素对ERE的上调作用。松果菊苷和毛蕊花糖苷通过上调ERE荧光素酶活性而表现出植物雌激素活性，可作为候选SERMs。

关键词: 松果菊苷, 毛蕊花糖苷, 植物雌激素, 雌激素受体, SERMs

Abstract: In this study,the estrogenic effects of echinacoside and actsoside of Herba Cistanche was investigated.A luciferase reporter plasmid law was used to detect the estrogenic effects of echinacoside and actsoside.The estrogen receptor competition binding experiments were used to detect their competitive effect with estrogen.The results showed that echinacoside and actsoside can increase the activity of ERE.When echinacoside or actsoside added in estrogenacts,EREdriven reporter gene activity can be stimulated as an estrogen agonist.Hence,it was concluded that echinacoside and actsoside had a phytoestrogens activity through stimulating ERE-increase reporter gene activity.Echinacoside and actsoside were potential SERMs.

Key words: echinacoside, actsoside, phytoestrogens, estrogen receptor, SERMs

中图分类号:

S285.5

王琳琳，李薇，宋新波*，余河水，苗琳，张丽娟，陶蕊. 肉苁蓉中松果菊苷和毛蕊花糖苷的植物雌激素活性研究[J]. 天然产物研究与开发, 2015, 27(3): 377-380.

WANG Lin-lin, LI Wei, SONG Xin-bo*, YU He-shui, MIAO Lin, ZHANG Li-juan, TAO Rui. Study on Estrogenic Effects of Echinacoside and Actsoside from Herba Cistanche[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(3): 377-380.

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肉苁蓉中松果菊苷和毛蕊花糖苷的植物雌激素活性研究

Study on Estrogenic Effects of Echinacoside and Actsoside from Herba Cistanche

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