青风藤醇洗脱液与青藤碱治疗关节炎大鼠比较研究

doi:10.16333/j.1001-6880.2015.03.027

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (3): 502-505.doi: 10.16333/j.1001-6880.2015.03.027

所属专题： No.5

青风藤醇洗脱液与青藤碱治疗关节炎大鼠比较研究

徐佳1，姚瑶2，葛卫红2*

1 南京中医药大学中西医结合鼓楼临床医学院；2 南京大学医学院附属鼓楼医院，南京210008

出版日期:2015-03-30 发布日期:2015-04-07

A Comparative Study of Sinomenium acutum Ethanol Eluent and Sinomenine Treat Arthritis in Rats

XU Jia1,YAO Yao2,GE Wei-hong2*

1 Department of Pharmacy,Drum Tower Medical College of Chinese and Western Integrative Medicine,Nanjing University of Chinese Medicine; 2 Department of Pharmacy,the Affiliated Drum Tower Hospital to Nanjing University Medical School,Nanjing 210008,China

Online:2015-03-30 Published:2015-04-07

摘要/Abstract

摘要： 观察青风藤大孔树脂95%乙醇洗脱物对大鼠胶原诱导性关节炎（CIA）体内相关炎症因子的表达，比较青风藤醇洗脱物与青藤碱单体对类风湿性关节炎的疗效。建立大鼠CIA模型进行关节评分，持续给药，抽取各组大鼠血样样本，ELISA法测定各组大鼠血清中IL-17、RANKL、OPG水平。青藤碱能够提高OPG水平，而青风藤95%乙醇洗脱物对RANKL、IL-17分泌的抑制作用优于青藤碱，提示青风藤95%乙醇洗脱物中可能存在其他青藤碱类似物，亦能发挥抗炎抗骨破坏的作用，青风藤95%乙醇洗脱物成分分析和具体机制仍有待进一步研究。

关键词: 青风藤醇洗脱物, 青藤碱, 核因子&kappa, B配位体受体活化剂, 可溶性蛋白骨保护素, 白细胞介素-17

Abstract: The aim of this study was to investigate the effect of macroporous resin 95% ethanol eluent of Sinomenium acutum on the expression of inflammatory in collagen-induced arthritis (CIA) rat,and to compare the effect of S.acutum alcohol eluent and sinomenine on rheumatoid arthritis of rats.The CIA rat model was established. The joint score,continued administration,drawn blood samples of rats were estimated and then the IL-17,RANKL,OPG levels were measured in serum by ELISA.Sinomenine can increase OPG levels,but the inhibitory effect of macroporous resin 95% ethanol eluent of S.acutum on secretion of RANKL and IL-17 was better than sinomenine.It suggested that there might be some other sinomenine analogues in the 95% ethanol eluent of S.acutum which also played a role of anti-inflammatory and anti-bone destruction.The composition analysis of 95% ethanol eluent of S.acutum and specific mechanism need to be carried out in future.

Key words: Sinomenium acutum alcohol eluent, sinomenine, RANKL, OPG, IL-17

中图分类号:

R965.2

徐佳1，姚瑶2，葛卫红2*. 青风藤醇洗脱液与青藤碱治疗关节炎大鼠比较研究[J]. 天然产物研究与开发, 2015, 27(3): 502-505.

XU Jia1,YAO Yao2,GE Wei-hong2*. A Comparative Study of Sinomenium acutum Ethanol Eluent and Sinomenine Treat Arthritis in Rats[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(3): 502-505.

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青风藤醇洗脱液与青藤碱治疗关节炎大鼠比较研究

A Comparative Study of Sinomenium acutum Ethanol Eluent and Sinomenine Treat Arthritis in Rats

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