天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (6): 1099-1104.doi: 10.16333/j.1001-6880.2015.06.029

• 开发研究 • 上一篇    下一篇

响应面法优化蕨麻多糖的提取工艺

高丹丹*,杨璐璐,祁高展   

  1. 西北民族大学生命科学与工程学院,兰州 730030
  • 出版日期:2015-06-30 发布日期:2015-07-10

Optimization of Extraction Conditions of Polysaccharide from Potentilla anserina by Response Surface Methodology

GAO Dan-dan*, YANG Lu-lu,QI Gao-zhan   

  1. College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,China
  • Online:2015-06-30 Published:2015-07-10

摘要: 采用水提醇沉法对蕨麻多糖进行提取,并对提取工艺条件进行了研究。在单因素实验的基础上,以多糖提取率为评价指标,选取液料比、浸提时间、浸提温度三个因素作为响应因子,用Box-Behnken中心组合试验设计原理优化蕨麻多糖的提取工艺。最后得到蕨麻多糖热水浸提的最佳工艺条件为:液料比20.3:1,浸提时间4.37 h,浸提温度82 ℃。在此条件下蕨麻多糖的实际提取率可达到24.53±0.24%。

关键词: 蕨麻, 多糖, 提取, 响应面, 优化

Abstract: The extraction technology of the water-soluble polysaccharide from Potentilla anserina was studied in this paper.The influences of liquid-solid ratio (V/W),extraction time and temperature on the extraction yield were investigated.On the basis of single factor tests,a Box-Behnken design (BBD) was used to study the influence of the 3 independent variables on the extraction yield.The results showed that the optimal extraction parameters were as follows:the liqud-solid ratio was 20.25:1, extraction time was 4.37 h and extraction temperature was 82 ℃.The extraction yield of polysaccharide was 24.53±0.24%.

Key words: Potentilla anserine, polysaccharide, extraction, response surface, optimization

中图分类号: 

TS255.1