Folin-Ciocalteu比色法测定铜藻中褐藻多酚含量的研究

doi:10.16333/j.1001-6880.2015.08.011

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (8): 1374-1378.doi: 10.16333/j.1001-6880.2015.08.011

Folin-Ciocalteu比色法测定铜藻中褐藻多酚含量的研究

段海霞，林云霞，张金荣*，严小军，骆其君

宁波大学海洋学院教育部应用海洋生物技术重点实验室，宁波 315211

出版日期:2015-08-29 发布日期:2015-09-01

Determination of Phlorotannins in Sargassum horneri Using Folin-Ciocalteu Colorimetric Method

DUAN Hai-xia,LIN Yun-xia,ZHANG Jin-rong*,YAN Xiao-jun,LUO Qi-jun

Key Laboratory of Applied Marine Biotechnology,Ministry of Education,School of Marine Sciences,Ningbo University,Ningbo 315211,China

Online:2015-08-29 Published:2015-09-01

摘要/Abstract

摘要： 以没食子酸为标准品，使用福林酚（Folin-Ciocalteu）试剂，通过紫外-可见分光光度法，对铜藻中褐藻多酚的含量进行了测定。结果表明，测定波长为760 nm，显色试剂Folin-Ciocalteu的用量为1.25 mL，0.1 g/mL Na2CO3溶液用量为4.0 mL，定容至10 mL，显色温度为30 ℃，反应时间为60 min，多酚含量在15~50 μg/mL范围内与吸光度呈良好线性关系，回归方程为Y = 0.0138X+0.0267，R²=0.9993。本测定方法的平均回收率为96.18%，相对标准偏差（RSD）为2.44%。该方法的稳定性、重现性、精密度以及加标回收率的相对标准偏差均符合要求，可用于铜藻中褐藻多酚含量的测定。按照本方法测定铜藻中褐藻多酚的平均含量为17.36 mg/g（RSD = 1.25 %）。

关键词: 铜藻, 褐藻多酚, 福林酚

Abstract: With gallic acid as the standard,the content of phlorotannins in Sargassum horneri was determined by UV-visible spectrophotometric method using Folinphenol (Folin-Ciocalteu).The detection wavelength was set at 760 nm.The color reaction was carried out with 1.25 mL of Folin-Ciocalteu reagent and 4.0 mL of 0.1 g/mL aqueous Na₂CO₃ solution in darkness at 30 ℃ for 60 min.The linear range of the established standard curve was 15-50 μg/mL and the regression equation was Y=0.0138X+0.0267,R²=0.9993.The average recovery rate of the added sample was 96.18%,with relative standard deviation (RSD) of 2.44%.The RSDs of stability,reproducibility,precision and recovery rate of the developed method were all satisfactory.Thus,this method can be applied to determine phlorotannins of S.horneri. The average content of phlorotannins in S.horneri was 17.36 mg/g (RSD=1.25%).

Key words: Sargassum horneri, phlorotannins, Folin-Ciocalteu reagent

中图分类号:

R284.1

段海霞，林云霞，张金荣*，严小军，骆其君 . Folin-Ciocalteu比色法测定铜藻中褐藻多酚含量的研究[J]. 天然产物研究与开发, 2015, 27(8): 1374-1378.

Folin-Ciocalteu比色法测定铜藻中褐藻多酚含量的研究

Determination of Phlorotannins in Sargassum horneri Using Folin-Ciocalteu Colorimetric Method

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