虾青素对运动性肾缺血再灌注损伤ECM表达的影响

doi:10.16333/j.1001-6880.2015.11.007

摘要/Abstract

摘要： 本文对虾青素对运动性肾缺血再灌注损伤大鼠肾组织炎症因子及ECM表达的影响进行了研究。72只SPF级Wistar大鼠随机分为4组：安静对照组（C组，n=12）、一般训练组（M组，n=12）、过度训练组（OM组，n=24）和虾青素+过度训练组（AM组，n=24）。实验中对大鼠以20 mg/(kg·d)虾青素灌胃56 d，并进行递增负荷游泳训练。末次训练24 h后观察肾小球ECM沉积情况并测试血清尿素氮、肌酐及肾组织炎症因子蛋白和基因表达、TGF-β1基因表达等生化指标。结果显示，8周的训练导致大鼠运动性肾缺血再灌注，ECM沉积加强，肾功能损伤。肾小球ECM沉积，C、M组间无显著差异（P>0.05）；OM组较C、M组显著增加（P<0.01）；AM组显著低于OM组（P<0.05）。血清尿素氮和肌酐水平，OM组和AM组显著高于C组（P<0.01），AM组显著低于OM组（P<0.05）；肾组织TNF-α、IL-1β、IL-6和IL-18蛋白表达，OM组和AM组显著高于C组（P<0.05或P<0.01），AM组显著低于OM组（P<0.05）；肾组织TNF-α mRNA、IL-1β mRNA、IL-6 mRNA和IL-18 mRNA表达，OM、AM组显著高于C组（P<0.01），AM组显著低于OM组（P<0.05）；肾组织TGF-β1 mRNA表达，AM组（P<0.05）和OM组（P<0.01）显著高于C组，AM组显著低于OM组（P<0.05）。从而说明补充虾青素可以有效地抑制肾组织中炎症因子表达从而减轻过度训练诱导的运动性肾脏缺血再灌注发生时肾脏组织TGF-β1的表达，维护ECM的动态平衡，延缓或避免对肾脏的损伤。

关键词: 虾青素, 运动性肾缺血再灌注损伤, 炎症因子, TGF-&beta, 1, 细胞外基质

Abstract: The aim of study was to investigate the protective effects of astaxanthin on kidney inflammation factors and extracellular matrix (ECM) expression in exercise-related renal ischemia-reperfusion injury rats.72 SPF Wistar rats were randomly divided into 4 groups:control group (C group,n=12),general training group (M group, n=12), overtraining group (OM group, n=24) and astaxanthin + overtraining group (AM group, n=24). During the experiment,the rats were given astaxanthin,gavages (ig) dose was 20 mg/kg per day.The rats were assigned to a 56 day increasing load swimming training.24 hours after the last training, we observed the glomerular ECM deposition,the serum urea nitrogen and creatinine,the expression of inflammatory cytokines protein and gene,TGF-β1 gene in renal tissue.The results showed that 8 weeks of training caused exercise-induced renal ischemia reperfusion in rats,with the increasement of glomerular ECM deposition and the impairment of renal function.The glomerular ECM deposition of C group and M group appear no differences (P>0.05);OM group was significantly higher than that in C group and M group (P< 0.01);AM group was significantly lower than that of OM group (P< 0.05).The serum urea nitrogen and creatinine levels of OM group and AM group were significantly higher than C group (P<0.01),AM group was significantly lower than OM group (P<0.05);the expression of renal TNF-α, IL-1β, IL-6 and IL-18 protein in OM group and AM group were significantly higher than C group (P<0.05 or P<0.01),AM group was significantly lower than OM group (P<0.05);the expression of renal TNF-α mRNA, IL-1β mRNA, IL-6 and IL-18 mRNA in OM group and AM group were significantly higher than C group (P<0.01),AM group was significantly lower than OM group (P<0.05);the expression of renal TGF-β1 mRNA in AM group (P<0.05) and OM group (P<0.01) was significantly higher than C group,AM group was significantly lower than OM group (P<0.05).To conclude,the supplement of astaxanthin can effectively inhibit the expression of inflammatory cytokines in renal tissue thereby reducing the expression of TGF-β1 in renal tissue when overtraining-induced renal ischemia-reperfusion occurs,maintain ECM homeostasis,delay or avoid the injury of renal.

Key words: astlxanthin, exercise-related renal ischemia-reperfusion injury, inflammation factors, TGF-&beta, 1, extracellular matrix

中图分类号:

G804.7

周海涛，曹建民2，郭娴2，龚平1*，冯玮2，王灿2，万子一1. 虾青素对运动性肾缺血再灌注损伤ECM表达的影响[J]. 天然产物研究与开发, 2015, 27(11): 1871-1877.

ZHOU Hai-tao1, CAO Jian-min2, GUO Xian2, GONG Ping1*, FENG Wei2, WANG Can2, WAN Zi-yi1. Effect of Astaxanthin on Kidney ECM Expression in Exercise-related Renal Ischemia-reperfusion Injury Rats[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(11): 1871-1877.

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虾青素对运动性肾缺血再灌注损伤ECM表达的影响

Effect of Astaxanthin on Kidney ECM Expression in Exercise-related Renal Ischemia-reperfusion Injury Rats

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