拟康氏木霉菌YIM PH30002铁载体活性化学成分

doi:10.16333/j.1001-6880.2015.11.008

天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (11): 1878-1883.doi: 10.16333/j.1001-6880.2015.11.008

拟康氏木霉菌YIM PH30002铁载体活性化学成分

陈金莲1,2，刘凯2，苗翠萍2，官会林1，赵立兴2*，孙世中1*

1 云南师范大学能源与环境科学学院，昆明 650092；2 云南大学省微生物研究所教育部微生物多样性可持续利用重点实验室，昆明 650091

出版日期:2015-11-30 发布日期:2015-12-02

Chemical Constituents with Siderophores Activities from Trichoderma koningiopsis YIM PH30002

CHEN Jin-lian1,2, LIU Kai2, MIAO Cui-ping2, GUAN Hui-lin1, ZHAO Li-xing2*, SUN Shi-zhong1*

1 College of Energy and Environmental Science,Yunnan Normal University,Kunming 650092,China; 2 Key Laboratory of Microbial Diversity in Southwest China of Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources,Yunnan Institute of Microbiology,Yunnan University,Kunming 650091,China

Online:2015-11-30 Published:2015-12-02

摘要/Abstract

摘要： 通过双层琼脂平板法对拟康氏木霉菌YIM PH30002在三种培养基条件下产铁载体能进行了半定量分析检测，该菌株在PDB培养基中，产铁载体成分的量最多。利用多种色谱技术从菌株YIM PH30002的PDB发酵液的乙酸乙酯提取物中共获得了5个聚酮类代谢产物，经波谱数据分析与文献比对，化合物分别鉴定为：konginginin A （1）、konginginin B （2）、konginginin D （3）、konginginin F （4）、konginginin M （5）。化合物1~4表现有铁载体活性，其中化合物2的铁载体活性相对较强，MTC值约为300 μg/mL。但化合物均无显著抗菌生物活性。

关键词: 拟康氏木霉, 铁载体活性, 聚酮化合物

Abstract: The semiquantitative analysis of siderophore produced by Trichoderma koningiopsis YIM PH30002 were detected by double-layer agar plate method.The results indicated the PDB medium was suitable for the strain YIM PH30002 to produce siderophore with maximum production.With further investigation,five polyketides were isolated from the ethyl acetate extract of the PDB fermentation broth of strain YIM PH30002 by various column chromatographical techniques.Their structures were elucidated as konginginin A (1),konginginin B (2),konginginin D (3),konginginin F (4),konginginin M (5) on the basis of spectroscopic analysis and literature data.Compounds 1-4 showed siderophore activities,among them compound 2 presented higher activity with MTC of 300 μg/mL in the iron (Fe III) acquisition tests.However,compounds 1-5 exhibited weak antimicrobial activities.

Key words: Trichoderma koningiopsis, siderophores, polyketides

中图分类号:

R284.2 O629.9

陈金莲1,2，刘凯2，苗翠萍2，官会林1，赵立兴2*，孙世中1*. 拟康氏木霉菌YIM PH30002铁载体活性化学成分[J]. 天然产物研究与开发, 2015, 27(11): 1878-1883.

CHEN Jin-lian1,2, LIU Kai2, MIAO Cui-ping2, GUAN Hui-lin1, ZHAO Li-xing2*, SUN Shi-zhong1*. Chemical Constituents with Siderophores Activities from Trichoderma koningiopsis YIM PH30002[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(11): 1878-1883.

拟康氏木霉菌YIM PH30002铁载体活性化学成分

Chemical Constituents with Siderophores Activities from Trichoderma koningiopsis YIM PH30002

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