关键词: DDAH ₂, 苦荞麦总黄酮, 脐静脉内皮细胞, NO

Abstract: To observe the effects of total flavonoids of Fagopyrum tataricum (L.) Gaertn (tartary buckwheat) on DDAH₂ in EA.hy926 cells in the presence of palmitic acid (PA),EA.hy926 cells were divided into control group,insulin resistance group,tartary buckwheat total flavonoids group and metformin group.Each group were combined insulin (50 nmol/L).Compared with control group,other groups were combined palmitic acid (600 μmol/L).Tartary buckwheat total flavonoids group was combined total flavonoids of tartary buckwheat (125 mg/L).Metformin group was combined metformin (2 mmol/L).The contents of ADMA were tested by double antibody sandwich method.The contents of NO were tested by nitrate reductase method.The DDAH₂ mRNA and protein expression levels were determined by RT-PCR and Western-blot assays,respectively.Compared with insulin resistance group,the content of ADMA of middle and high dose groups were significantly reduced,the content of NO were increased significantly (P<0.05).Compared with insulin resistance group,the expression levels of DDAH₂mRNA and protein were significantly higher in tartary buckwheat group (P<0.05).Total flavonoids of tartary buckwheat promoted the expression of DDAH₂ mRNA and protein effectively in EA.hy926 cells under PA stimulation

Key words: DDAH₂, total flavonoids of tartary buckwheat, EA.hy926, NO