砂生槐多糖提取物对免疫抑制模型小鼠Treg和Th17的影响

doi:10.16333/j.1001-6880.2016.10.008

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (10): 1545-1548.doi: 10.16333/j.1001-6880.2016.10.008

砂生槐多糖提取物对免疫抑制模型小鼠Treg和Th17的影响

雒艳萍1，贾天玉1，管彬1，冯燕海1，史彦斌2，马兴铭1,3*

1 兰州大学基础医学院；2 兰州大学药学院；3甘肃省新药临床前研究重点实验室，兰州 730000

出版日期:2016-10-31 发布日期:2017-03-30

Effects of Polysaccharides Extract of Sophora moorcroftiana on CD4+CD25+Treg and Th17 in Immunosuppressed Mice

LUO Yan-ping1,JIA Tian-yu1,GUAN Bin1,FENG Yan-hai1,SHI Yan-bin2,MA Xing-ming1,3*

1 School of Basic Medical Sciences,Lanzhou University;2School of Pharmacy,Lanzhou University;3 Key Lab of Preclinical Study for New Drugs of Gansu Province,Lanzhou 730000,China

Online:2016-10-31 Published:2017-03-30

摘要/Abstract

摘要： 为了开发藏药砂生槐的药用价值，本文拟研究其多糖提取物对免疫抑制小鼠调节性T细胞（Treg）和Th17的影响。水提醇沉法提取砂生槐枝条多糖物质，腹腔注射环磷酰胺建立免疫抑制小鼠模型，模型小鼠经砂生槐多糖灌胃干预后，分离脾细胞，流式细胞术检测CD4+T细胞比例和CD4+CD25+Treg细胞比例；收集血清，ELISA检测IL-17水平。结果显示，各剂量组砂生槐多糖均能够引起免疫抑制小鼠的脾脏CD4+CD25+Treg细胞比例明显增多（P<0.05），同时明显抑制IL-17的产生（P<0.05）。以上结果提示砂生槐多糖可能通过上调Treg而抑制免疫功能，同时抑制IL-17的产生，发挥免疫抑制作用。

关键词: 砂生槐, 植物多糖, 调节性T细胞, IL-17

Abstract: Sophora moorcroftiana is the specific plant in Tibet and gets little development since grows in the remote place.In order to research its medicinal value,we firstly extract Sophora moorcroftiana polysaccharides (SMP) and investigate the effects on CD4+CD25+ regulatory T cells (CD4+CD25+Treg) cells and Th17 cells in immunosuppressed mice.SMP was extracted from branch of Sophora moorcroftiana after water extraction and alcohol precipitation.The immunosuppressed mice induced by cyclophosphamide were treated with SMP,then the ratio of CD4+T cells and CD4+CD25+Treg cells were analyzed by flow cytometry and the serum interleukin-17 (IL-17) were detected by enzyme-linked immunosorbent assay (ELISA).The results showed that each dose SMP increased the frequency of CD4+CD25+Treg in spleen and decreased the serum IL-17 production.In conclusion,SMP plays a role in immunosuppression by up-regulating Treg and suppressing IL-17 production.

Key words: Sophora moorcroftiana, Plant polysaccharide, Regulatory T cells, Interleukin-17

中图分类号:

R392 R932

雒艳萍1，贾天玉1，管彬1，冯燕海1，史彦斌2，马兴铭1,3*. 砂生槐多糖提取物对免疫抑制模型小鼠Treg和Th17的影响[J]. 天然产物研究与开发, 2016, 28(10): 1545-1548.

LUO Yan-ping1,JIA Tian-yu1,GUAN Bin1,FENG Yan-hai1,SHI Yan-bin2,MA Xing-ming1,3*. Effects of Polysaccharides Extract of Sophora moorcroftiana on CD4+CD25+Treg and Th17 in Immunosuppressed Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(10): 1545-1548.

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砂生槐多糖提取物对免疫抑制模型小鼠Treg和Th17的影响

Effects of Polysaccharides Extract of Sophora moorcroftiana on CD4+CD25+Treg and Th17 in Immunosuppressed Mice

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