天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (6): 874-879.doi: 10.16333/j.1001-6880.2016.6.011

• 研究论文 • 上一篇    下一篇

UPLC同时测定杜仲中6种有效成分的含量

孙佳1,2，陆苑1,2，向文英1,3，曹旭1,2，巩仔鹏1,2，王爱民1,3*   

  1. 1 贵州医科大学药学院；2 贵州省药物制剂重点实验室；3 民族药与中药开发应用教育部工程研究中心，贵阳 550004
  • 出版日期:2016-06-30 发布日期:2017-01-17

Simultaneous Determination of Six Components in Eucommia ulmoides by UPLC

SUN Jia1,2,LU Yuan1,2,XIANG Wen-ying1,3,CAO Xu1,2,GONG Zi-peng1,2,WANG Ai-min1,3*   

  1. 1 School of Pharmacy,Guizhou medical university; 2 Guizhou Provincial Key Laboratory of Pharmaceutics; 3 Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education),Guiyang 550004,China
  • Online:2016-06-30 Published:2017-01-17

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摘要： 建立UPLC同时测定杜仲药材中桃叶珊瑚苷、京尼平苷酸、绿原酸、京尼平苷、松脂醇二葡萄糖苷、松脂醇单葡萄糖苷的分析方法。以Waters BET C-18（2.1×150 mm，1.7 μm）为色谱柱，0.1%甲酸乙腈溶液-0.1%甲酸水溶液进行梯度洗脱，检测波长为205 nm，流速0.25 mL/min，柱温45 ℃。在该色谱条件下，杜仲中的六种有效成分能得到较好的分离，方法的加样回收率为96.92%~101.01%，RSD<3%。39批杜仲药材中六个被测成分的总含量0.4406%~4.2282%，差异较大。该方法简便、准确，重复性好，可用于杜仲中桃叶珊瑚苷、京尼平苷酸、绿原酸、京尼平苷、松脂醇二葡萄糖苷、松脂醇单葡萄糖苷的含量测定，为杜仲的质量控制研究提供依据。

关键词: 杜仲, 超高效液相色谱, 桃叶珊瑚苷, 绿原酸, 京尼平苷酸, 京尼平苷, 松脂醇二葡萄糖苷, 松脂醇单葡萄糖苷

Abstract: To establish an UPLC method for simultaneous determination of six compounds,namely aucubin,geniposidic acid,chlorogenic acid,geniposide,pinoresinol diglucoside and pinoresinol singleglucoside in Eucommia ulmoides.UPLC analysis was carried out on Waters BET C18 column (2.1×150 mm,1.7 μm),with the mobile phase of 0.1% formic acid acetonitrile solution 0.1% formic acid aqueous solution in gradient elution mode.The flow rate was 0.25 mL/min.The UV detection wavelength was 205 nm.The column temperature was 45 ℃.The six components of E.ulmoides can be separated in the chromatographic conditions.The recoveries of six components were 96.92%-101.01%,with RSD less than 3%.The total content of six components from 39 batch E.ulmoides were 0.4406%-4.2282%.The developed UPLC method was simple,accurate and highly reproducible.It can be used for the simultaneous detection and determination of aucubin,geniposidic acid,chlorogenic acid,geniposide,pinoresinol diglucoside and pinoresinol singleglucoside.

Key words: Eucommia ulmoides, UPLC, aucubin, geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, pinoresinol singleglucosid

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R284.1

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