荷包地不容生物碱成分研究及3种阿朴菲类生物碱的毒性初步评价

doi:10.16333/j.1001-6880.2016.7.001

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (7): 991-995.doi: 10.16333/j.1001-6880.2016.7.001

• 研究论文 • 下一篇

荷包地不容生物碱成分研究及3种阿朴菲类生物碱的毒性初步评价

晏菲1,2，马云淑1*，汪波1，陈成1，王辉1

1云南中医学院药学院，昆明 650500；2陕西中医药大学药学院，西安 712046

出版日期:2016-07-30 发布日期:2017-01-17

Isolation and Preliminary Toxicity Evaluation of Alkaloids from Stephania dicentrini fera H.S.Lo & M.Yang

YAN Fei1,2,MA Yun-shu1*,WANG Bo1,CHEN Cheng1,WANG Hui1

1 College of Pharmacy,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China; 2 College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China

Online:2016-07-30 Published:2017-01-17

摘要/Abstract

摘要： 采用硅胶柱色谱分离技术，结合TLC、紫外光谱、NMR、MS等方法对荷包地不容（Stephania.dicentrinifera H.S.Lo& M.Yang）中生物碱成分进行研究。采用小鼠尾静脉注射法进行同亚属植物中所得三种阿朴菲类（Aporphine，AP）生物碱的急性毒性试验，用Bliss法计算半数致死量（LD₅₀）并比较几种AP类生物碱的毒性与结构的关系。结果分离和鉴定了3个生物碱成分，分别为AP型的荷包牡丹酮（Dicentrinone，Dico）和荷包牡丹碱（Dicentrine，Dic），以及原小檗碱（Protoberberine，PBB）型的四氢巴马汀（Tetrahydropalmatine，Tet）。其中Dico为首次从荷包地不容中分离出来，其LD₅₀=7.266 mg/kg ，Dic的 LD₅₀=26.802 mg/kg，异可利定（Isocorydine，Isoc）的 LD₅₀=32.167 mg/kg。

关键词: 荷包地不容, 阿朴菲类生物碱, 荷包牡丹酮, 荷包牡丹碱, 异可利定, LD₅₀

Abstract: In this study,aporphine(AP)-type alkaloids were isolated from Stephania dicentrinifera H.S.Lo & M.Yang by silica gel column chromatography.Their structures were identified by TLC,UV,NMR,MS, etc.The acute toxicity of 3 obtained alkaloids ［Dicentrinone(Dico)and Dicentrine(Dic)of AP-type alkaloids, Tetrahydropalmatine(Tet) of Protoberberine(PBB)-type alkaloids］ was then investigated using the method of tail vein injection in mice,their LD₅₀'s were calculated by Bliss method.The experimental results showed that the LD₅₀ values of Dico,Dic and Isocorydine(Isoc)were 7.266 mg/kg,26.802 mg/kg and 32.167 mg/kg,respectively.Dico was separated from S.dicentrinifera for the first time,and its acute toxicity was higher than Dic and Isoc.

Key words: Stephania.dicentrinifera H.S.Lo &, M.Yang;aporphine alkaloids;dicentrinone;dicentrine;isocorydine;LD_50

中图分类号:

R28

晏菲1,2，马云淑1*，汪波1，陈成1，王辉1. 荷包地不容生物碱成分研究及3种阿朴菲类生物碱的毒性初步评价[J]. 天然产物研究与开发, 2016, 28(7): 991-995.

YAN Fei1,2,MA Yun-shu1*,WANG Bo1,CHEN Cheng1,WANG Hui1. Isolation and Preliminary Toxicity Evaluation of Alkaloids from Stephania dicentrini fera H.S.Lo & M.Yang[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(7): 991-995.

荷包地不容生物碱成分研究及3种阿朴菲类生物碱的毒性初步评价

Isolation and Preliminary Toxicity Evaluation of Alkaloids from Stephania dicentrini fera H.S.Lo & M.Yang

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