天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1752-1758.doi: 10.16333/j.1001-6880.2017.10.020

• 开发研究 • 上一篇    下一篇

多元素分析结合多元统计分析用于丹参产地溯源研究

赵恒强1,张敏敏1,赵志国1,耿岩玲1,黄璐琦2,王晓1*   

  1. 1山东省中药质量控制技术重点实验室 山东省科学院中药过程控制研究中心 山东省分析测试中心,济南250014;2中国中医科学院中药资源研究中心 道地药材国家重点实验室培育基地 中国中医科学院,北京100700
  • 出版日期:2017-10-27 发布日期:2017-10-27
  • 基金资助:

    中央本级重大增减支项目(2060302);国家自然基金重点项目(81130070)

Determination of Geographical Origin of Salvia miltiorrhiza by Multi-element Analysis Combined with Multivariate Statistical Analysis

ZHAO Heng-qiang1, ZHANG Min-min1, ZHAO Zhi-guo1, GENG Yan-ling1, HUANG Lu-qi2, WANG Xiao1*   

  1. 1Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Process Control Research Center of Traditional Chinese Medicine of Shandong Academy of Sciences, Jinan 250014,China; 2National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Online:2017-10-27 Published:2017-10-27

摘要: 本文旨在探讨采用多元素分析结合多元统计分析用于丹参产地溯源的可行性。采用ICP-MS测定丹参药材中的35种微量元素和稀土元素,采用主成分分析和线性判别分析用于不同省份丹参药材的判别。研究表明,其中18种元素表现出明显的差异(P<0.05);PCA法能够区分不同产地丹参,但存在一定重合;采用LDA分析可以实现不同产地丹参药材的正确判别,回判正确率为100%,交叉验证成功率为85.5%。该方法采用微量元素和稀土元素结合多元统计分析法可以在一定程度上实现不同产区丹参药材的正确区分,为中药产地溯源提供了方法支持。

关键词: 丹参, 产地溯源, 多元素分析, 多元统计分析

Abstract: To explore the feasibility for the discrimination of geographical origin of Salvia miltiorrhiza Bge.by multi-element analysis combined with multivariate statistical analysis.A total of 35 metal elements including rare earth elements were analyzed by ICP-MS.Multivariate statistical analysis including principal component analysis (PCA) and linear discriminate analysis (LDA) were used for the discrimination of S.miltiorrhiza  from different geographical origins.As a result,eighteen elements showed significant differences (P<0.05).LDA gave an overall correct classification rate of 100.0% and cross-validation rate of 85.5%.Therefore,multi-element fingerprinting combined with multivariate statistical analysis can be considered as a powerful tool for authenticating the geographical origin of traditional Chinese medicine.

Key words: Salvia miltiorrhiza Bge., geographical origin, multi-element analysis, multivariate statistical analysis

中图分类号: 

R917