关键词: 对羟基苯甲醛, OGD/Rep, 原代皮层神经元, 钙通道

Abstract: The rat primary cortical neurons oxygen-glucose deprivation/reintroduction (OGD/Rep) injury model was duplicated and was used to study the inhibitory effect of 4-hydroxybenzaldehyde on calcium channel.The cell viability was texted by MTT assay.The NO release was texted by nitric acid reductase assay.Intracellular calcium concentration was texted by flow cytometry and the protein and mRNA expressions of L-type calcium channel,NMDA receptor-type calcium channel and TRPM7 channel were texted by Western Blot and RT-PCR.The results indicated 4-hydroxybenzaldehydecan elevate survival rate of the primary cortical neuron which were affected by OGD/Rep injury,decrease NO release,reduce intracellular calcium iron concentration of the primary cortical neurons,as well as lower L channel protein expression.

Key words: 4-hydroxybenzaldehyde, OGD/Rep, primary cortical neurons, calcium channel