天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (10): 1721-1727.doi: 10.16333/j.1001-6880.2018.10.010

• 研究简报 • 上一篇    下一篇

杜仲内生青霉菌GZWMJZ-068次生代谢产物研究

龚倩玉1,4,许言超1,4,左明星1,2,饶青1,2,周彦伶1,4,朱伟明1,3,林昌虎1,4*,王立平1,2*   

  1. 1省部共建药用植物功效与利用国家重点实验室 贵州医科大学;2贵州省中国科学院天然产物化学重点实验室 贵阳 550014;3中国海洋大学医药学院,青岛 266003;4贵州医科大学药学院,贵阳 550025
  • 出版日期:2018-10-31 发布日期:2018-11-01
  • 基金资助:

    贵州省“百人领军人才”计划;黔科合院士站([2015]4009号);黔科合体(Z字[2014]4007号)

Secondary Metabolites of Endophytic Penicillium mallochii GZWMJZ-068 from Eucommia ulmoides

GONG Qian-yu1,4,XU Yan-chao1,4,ZUO Ming-xing1,2,RAO Qing1,2,ZHOU Yan-ling1,4,ZHU Wei-ming1,3,LIN Chang-hu1,4*,WANG Li-ping1,2*   

  1. 1State Key Laborataory of Functions and Applications of Medicinal Plants, Guizhou Medical University;2Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China; 3School of Medicine and Pharmacy, Ocean University of China,
    Qingdao 266003,China; 4Department of Pharmacology,Guizhou Medical University, Guiyang 550025, China
  • Online:2018-10-31 Published:2018-11-01

摘要: 为获得活性微生物代谢产物,从贵阳杜仲的叶片中分离得到内生真菌GZWMJZ-068,发酵产物用乙酸乙酯提取,经硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱等进行分离纯化,得到15个单体化合物。运用核磁、质谱和比旋光度等方法鉴定了15个化合物的结构,即isochromophilones IV(1)、epi-isochromophilone III(2)、(2R,3S)-1-(4-羟基苯基)-2,3-丁二醇(3)、(2S,3S)-1-(4-羟基苯基)-2,3-丁二醇(4)、6-[1-羟基-(1S)-戊基]-4-甲氧基-(6S)-2H,5H-2-吡喃酮(5)、6-(1-羟戊基)-4-甲氧基-2-酮(6)、quinolactacins Al(7a)、quinolactacins A2(7b)、dihydrocitrinone(8)、dihydrocitrinin (9)、原儿茶酸(10)、儿茶酸 (11)、5-甲基尿嘧啶(12)、尿嘧啶(13)、citrinal A(14)。对15个化合物进行了抑菌活性评价,化合物1和2对光滑念珠菌和金黄色葡萄球菌具有中等的抑制作用(MIC16-32 μg/mL)。

关键词: 杜仲, 内生真菌, 代谢产物, 抑菌活性

Abstract: In order to investigate bioactive metabolites from endophytic fungi,a fungal GZWMJZ-068 was isolated and identified as Penicillium mallochii from the leaf of Eucommia ulmoides in Guiyang Guizhou,China.The extract of the fermentation was purified by column chromatography over silica gel,Sephadex LH-20 and semi-preparative HPLC.The structures were identified by means of NMR,MS and ORD.As a result,15 compounds,isochromophilones IV(1)、epi-isochromophilone III(2)、(2R,3S)-1-(4-Hydroxyphenyl)- butane-2,3-diol(3)、(2S,3S)-1-(4-Hydroxyphenyl)butane-2,3-diol(4)、6-[1-Hydroxy-(1S)-pentyl]-4-methoxy-(6S)-2H,5H-2-pyranone(5)、6-(1-hydroxypentyl)-4-methoxy-pyran-2-one(6)、quinolactacins Al(7a)、quinolactacins A2(7b)、dihydrocitrinone(8)、dihydrocitrinin(9)、protocatechuic acid(10)、pyrocatechuic acid(11)、5-Methyluracil(12)、uracil(13)、citrinal A(14).The antibacterial activities of 1-14 against 9 pathogenic microorganisms were tested by two-fold dilution method.Compounds 1 and 2 were active against Staphylococcus aureus and Candida glabrata with the minimum inhibitory concentration(MIC)values from 16 μg/mL to 32 μg/mL,respectively.

Key words: Eucommia ulmoides, endophytic fungi, secondary metabolites, antibacterial activities

中图分类号: 

R284.2