关键词: 蓝莓提取物, 超高效液相色谱－四级杆/飞行时间质谱, 花青素, 黄酮

Abstract: The anthocyanins and flavonoids ingredients in blueberry extracts from different manufacturers were analyzed under TOF/MS full scan mode and Q-TOF/MS fragment scan mode simultaneously using ultra-performance liquid chromatography/time-of-flight mass spectrometry.First of all,based on the molecular formula calculated by accurate mass,the possible compounds were found in the ‘lists of potential compounds’,then these compounds were qualitatively analyzed according to the fragmentation characteristics of MS².Combined with the references,19 anthocyanins and 8 flavonoids were identified from the blueberry extracts.At the same time,the present study also explored the fragmentation regularities of mass spectra of anthocyanin and flavonoid glycosides in blueberry extract.This method with the characteristics of fast,sensitivity,accuracy can be generalized to the screening of other natural active ingredients without reference.

Key words: blueberry extract, UPLC-ESI-Q-TOF/MS, anthocyanins, flavonoids