辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究

doi:10.16333/j.1001-6880.2018.6.023

天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (6): 1061-1065.doi: 10.16333/j.1001-6880.2018.6.023

辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究

阳凤，王瑶，尹德锋，廖文筠，彭林，伍洋，彭燕*

西南医科大学附属医院, 泸州 646000

出版日期:2018-07-05 发布日期:2018-07-05
基金资助:
四川省科技厅课题（12037）

The Effect and Mechanism of Capsaicin Prevented Acute Gastric Mucosal Injury by Gastric Ischemia-reperfusion

YANG Feng,WANG Yao,YIN De-feng,LIAO Wen-jun,PENG Lin,WU Yang,PENG Yan*

The Affiliated Hospital of Southwest Medical University,Sichuan Province 646000,China

Online:2018-07-05 Published:2018-07-05

摘要/Abstract

摘要： 本研究为探讨辣椒素（CAP）对大鼠胃缺血再灌注损伤（GI-R）的作用及机制。成年雄性健康SD大鼠40只，随机分为4组，每组10只，A组（空白对照组），B组（手术组），C组（CAP组），D组（CAP +手术组）。给予A组、B组大鼠灌胃生理盐水10 mL/kg/d，C组、D组大鼠灌胃CAP1 mg/kg/d。4周后B组、D组于GI-R模型建立后24小时处死大鼠，收集胃液测定胃酸总酸度，肉眼计数胃黏膜损伤指数，行胃窦组织病理学观察，检测胃黏膜辣椒素受体（TRPV1）、降钙素基因相关肽（CGRP）的表达，检测胃窦组织丙二醛（MDA）、超氧化物歧化酶（SOD）的水平。结果显示A组、C组胃黏膜未见损伤；与B组相比，D组胃黏膜损伤程度显著减轻（P＜0.05），胃酸总酸度显著降低（P＜0.05），MDA值显著降低（P＜0.05），SOD值显著升高（P＜0.05），TRPV1、CGRP表达显著增多（P＜0.05）。实验结果表明预先给予CAP 1 mg/kg/d 连续灌胃4周具有预防缺血再灌注致大鼠胃黏膜损伤的作用，其机制可能与CAP上调TRPV1、CGRP在胃内的表达、减轻氧自由基损伤有关。

关键词: 辣椒素, 胃缺血再灌注, 胃黏膜损伤, TRPV1, CGRP

Abstract: This study was to explore the effect and mechanism of capsaicin on gastric ischemia-reperfusion injury in rats.Specific methods were as follows:40 SD rats were randomly divided into 4 groups with 10 rats in each group，group A(control group),group B(operation group),group C(CAP group) and group D(CAP+operation group).Rats in group A and group B were treated with intragastric administration of normal saline 10 ml/kg/d,group C and group D with intragastric administration of CAP1mg/kg/d.After 4 weeks,the rats in group B and group D were killed 24 hours later after the establishment of GI-R model.Last,astric juice was collected to determine the total acidity of gastric acid,counted the gastric mucosal injury index,observed the gastric mucosa pathological injury,and detected the expression of TRPV1、CGRP、MDA、SOD.Results show that:the gastric mucosa of group A and C had no damage.Compared with group B,the gastric mucosa injury were significantly reduced（P＜0.05）,total acidity decreased significantly（P＜0.05）,MDA decreased significantly（P＜0.05）,SOD increased significantly（P＜0.05）and the expression of TRPV1、CGRP increased significantly（P＜0.05）in group D.Experimental results show that:it could prevent that GI-R induced acute gastric mucosal injury in rats by pretreated with CAP 1 mg/kg/d for 4weeks.The mechanism may be related to the up regulation of TRPV1 and CGRP expression in the stomach and the reduction of oxygen free radical damage by CAP.

Key words: CAP, GI-R , Gastric mucosal injury, TRPV1, CGRP

中图分类号:

Q939.9 R961

阳凤，王瑶，尹德锋，廖文筠，彭林，伍洋，彭燕*. 辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究[J]. 天然产物研究与开发, 2018, 30(6): 1061-1065.

YANG Feng,WANG Yao,YIN De-feng,LIAO Wen-jun,PENG Lin,WU Yang,PENG Yan*. The Effect and Mechanism of Capsaicin Prevented Acute Gastric Mucosal Injury by Gastric Ischemia-reperfusion[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2018, 30(6): 1061-1065.

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辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究

The Effect and Mechanism of Capsaicin Prevented Acute Gastric Mucosal Injury by Gastric Ischemia-reperfusion

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