天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (7): 1119-1125.doi: 10.16333/j.1001-6880.2018.7.004

• 研究论文 • 上一篇    下一篇

液相色谱-质谱联用技术分析提取温度对药材中人参皂苷单体提取率的影响

李慧,焦丽丽,章国磊,吴巍*,刘淑莹   

  1. 长春中医药大学 吉林省人参科学研究院 长春 130117
  • 出版日期:2018-08-08 发布日期:2018-08-08
  • 基金资助:

    吉林省科技发展计划(201603088YY),吉林省优秀青年人才基金(20180520062JH)

Temperature Influence on Extraction Efficiency of Ginseng Saponin with Liquid Chromatography-Mass Spectrometry

LI Hui,JIAO Li-li,ZHANG Guo-lei,WU Wei*,LIU Shu-ying   

  1. Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun,130117,China
  • Online:2018-08-08 Published:2018-08-08

摘要: 建立快速高分离度液相色谱-四级杆飞行时间质谱联用方法(LC-Q-TOF-MS)对人参皂苷进行定性和定量分析,鉴定了20种皂苷单体,以提取离子流峰面积定量分析,验证了含量测定方法学。并研究了温度对人参皂苷提取率的影响。总皂苷的提取效率随温度升高而逐渐增加,单体的变化趋势与总皂苷不相同:二醇型皂苷在100  ℃时提取率最高,三醇型皂苷在80 ℃左右出现最高点,并随着温度的进一步升高而降低,丙二酰基人参皂苷在70  ℃最高,高于70 ℃时发生降解。人参皂苷单体的提取效率与温度相关,提取过程皂苷发生降解。

关键词: 液质联用, 人参皂苷, 提取率

Abstract: A rapid,high-resolution liquid chromatography with quadrupole time of flight mass spectrometry (LC-Q-TOF-MS) was established for ginseng saponin analysis.Twenty saponin monomers were identified and quantitative analyzed with validated method.The method was applied for the investigation of temperature influence of the temperature on ginseng saponin extraction efficiency.The extracted ion chromatographic (EIC) peak area was used for quantitative analysis:the highest extraction efficiency of protopanaxadiol temperature was at 100  ℃,while the temperature for protopanaxatriol was at around 80 ℃,then decreased with increasing temperature.The highest extraction efficiency of malonyl-ginsenoside was at 70  ℃.However,results showed that total saplonin increased gradually with temperature,which could not represent the tendency of saponin monomers.The extraction efficiency of ginseng saponin was related to temperature,and the saponin was degraded during the extraction process.

Key words: LC/MS, ginseng saponin, extraction efficiency

中图分类号: 

R284.1 R285