关键词: 桑白皮, 桑根酮 B, RAW264.7细胞, 炎症, Diels-Alder加合物

Abstract: Cortex Mori was separated by silica gel column chromatography to afford the active compound.The structure elucidation of the compound was identified by analysis of spectroscopic data.RAW264.7 cells were stimulated by lipopolysaccharide (LPS) to induce inflammatory model.Cell viability was determined using a methyl thiazolyl tetrazolium (MTT) assay.The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α),interleukin 6 (IL-6) were measured by Griess reaction and ELISA,respectively.The inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA and protein levels were measured by qRT-PCR and Western blot,respectively.Results showed Cortex Mori ethanol extract (MRE) was able to inhibit the production of NO in LPS-stimulated RAW264.7 cells,in a dose-dependent manner.One active compound was isolated from Cortex Mori and identified as the Diels-Alder type adducts sanggenon B.The anti-inflammatory results showed that sanggenon B could significantly inhibit the production of NO,TNF-α,and IL-6.Sanggenon B also caused a significant dose-dependent decrease in the mRNA and protein expressions of iNOS,COX-2.In conclusion,sanggenon B isolated from Cortex Mori exhibited obvious anti-inflammatory effect and its mechanism may be related to its regulation on inflammatory cytokines,and inhibition of the expression of iNOS,COX-2.

Key words: Cortex Mori, sanggenon B, RAW264.7 cells, inflammation, Diels-Alder type adducts