关键词: 荷包山桂花, 远志科, 化学成分, 结构鉴定, 抗氧化

Abstract: To investigate the chemical constituents from the 75% alcohol fraction of the roots of Polygala arillata Buch.-Ham.ex D.Don.Column chromatographies of silica gel,preparative HPLC and Sephadex LH-20 were applied to isolate the chemical constituents.Pure compounds were elucidated by means of ¹H NMR,¹³C NMR and LC-MS.Ten compounds were isolated and identified as β-D-(3-O-sinapoyl)-fructofuranosyl-α-D -(6-O-sinapoyl)-glucopyranoside (1),cis-coniferin (2),α-spinasterol (3),2′-deoxythymidine (4),α-spinasteryl 3-O-β-D-glucopyranoside (5),β-sitosterol (6),glucosyringic acid (7),glucose (8),sucrose (9),p-hydroxybenzaldehyde (10).Compounds 2-5 and 7 were obtainted from Polygala arillata Buch.-Ham.ex D.Don for the first time.The antioxidant activities of compounds 1~7 and 10 were tested by using the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays,and only compound 1 exhibited moderate antioxidant activity compared to ascorbic acid.

Key words: Polygala arillata Buch.-Ham.ex D.Don, Polygalaceae, chemical constituents, structure elucidation, antioxidant