天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (1): 16-23.doi: 10.16333/j.1001-6880.2019.1.003

• 研究论文 • 上一篇    下一篇

基于近红外光谱技术的白茶可溶性糖总量快速测定研究

沈诗钰1,孙威江1,2,3,4*,唐琴1,陈佳佳1,林馥茗2,黄艳2,周喆1,陈翠翠2   

  1. 1福建农林大学园艺学院,福州 350002;2福建农林大学安溪茶学院,泉州 362400;3福建省茶产业技术开发基地;4福建省茶业工程技术研究中心,福州 350002;
  • 出版日期:2019-01-28 发布日期:2019-01-28
  • 基金资助:

    科技部重点研发专项“国家质量基础的共性技术研究与应用”子项(2016YFF0201904-03);福建省科技厅科技重大专项专题(2017NZ0002-1)

Rapid determination of total water-soluble sugars in white tea by near-infrared spectroscopy

SHEN Shi-yu1,SUN Wei-jiang2,3,4*,TANG Qin1,CHEN Jia-jia1,LIN Fu-ming2,HUANG Yan2,ZHOU Zhe1,CHEN Cui-cui2   

  1. 1Horticultural college of Fujian Agriculture and Forestry University,Fuzhou 350002,China;  2Anxi tea college of Fujian Agriculture and Forestry University,Fuzhou 362400,China; 3Tea Industry Technology Development Base of Fujian Province; 4Tea Industry Engineering Technology Research Center of Fujian Province,Fuzhou 350002,China
  • Online:2019-01-28 Published:2019-01-28

摘要: 本文以积分球漫反射模块采集113份不同等级不同年份的白茶近红外光谱图并进行预处理分析,采用蒽酮比色法对来自不同厂家的白茶进行含量测定,运用偏最小二乘法(PLS)建立了白茶可溶性糖总量快速测定模型并对模型进行验证。试验结果表明所建立模型的相关系数(R)为0.963,校正均方根差(RMSEC)为0.363 9,验证均方根差(RMSEP)为0.349,验证集平均相对误差为3.11%。通过NIRS快速测定白茶总糖含量具有较高的可行性,该方法预测结果较好,能够准确、快速、无损的对白茶可溶性糖总量进行快速定量分析。

关键词: 白茶, 可溶性糖总量, 快速测定, 近红外光谱技术

Abstract: This paper quantitatively determines the total water-soluble sugars in white tea rapidly by using Near-infrared Spectroscopy (NIRS) method.113 white tea samples,manufactured by distinct providers in different years,and of different quality grades,are used to do the determination experiments.The near-infrared spectra of these samples is acquired and analyzed in the first place.And then a quantitative analysis model of total water-soluble sugars in white tea is established by adopting partial least square (PLS) method.The experimental results show that the correlation coefficient (R) of the proposed quantitative analysis model is 0.963 9,while the root mean square error of calibration (RMSEC),the root mean square error of predication (RMSEP),and the average relative error of the validation set are 0.363,0.349 and 3.11%,respectively.The model is of good predication performance,and thus can quantitatively determinate the total water-soluble sugars in white tea accurately,rapidly and losslessly.The result indicates the feasibility of NIRS in rapid determination of total water-soluble sugars in white tea.

Key words: white tea, total water-soluble sugars, rapid determination, NIRS

中图分类号: 

Q946.9