天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (11): 1818-1828.doi: 10.16333/j.1001-6880.2021.11.002

• 研究论文 • 上一篇    下一篇

基于UPLC-QTOF-MS技术分析野生与栽培杨树桑黄的化学成分

昝立峰1,3*，范宇光2，包海鹰3，图力古尔3，叶嘉1   

  1. 1邯郸学院生命科学与工程学院，邯郸 056005；2海南医学院药学院，海口 571199；3吉林农业大学药用菌物资源及其开发利用重点研究室，长春 130118



  • 出版日期:2021-11-28 发布日期:2021-12-02
  • 基金资助:
    河北省自然科学基金资助项目（C2019109043）

Characterization of chemical compositions from the wild and cultivated Sanghuangporus vaninii based on UPLC-QTOF-MS

ZAN Li-feng1,3*,FAN Yu-guang2,BAO Hai-ying3,Tolgor3,YE Jia1   

  1. 1School of Life Science and Engineering,Handan University,Handan 056005,China; 2Pharmacy College,Hainan Medical University,Haikou 571199,China;3Key Laboratory of Medicinal Fungal Resources and Development and Utilization,Jilin Agricultural University,Changchun 130118,China
  • Online:2021-11-28 Published:2021-12-02

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摘要： 采用超高效液相色谱-串联四级杆-飞行时间质谱（UPLC-QTOF-MS）技术快速识别和鉴定了野生与栽培杨树桑黄子实体的化学成分。选用反相Waters Acouity UPLC BEH C18（100 mm×2.1 mm，1.7 μm）色谱柱，甲醇与水（含0.1%甲酸）作为流动相二元线性梯度洗脱，在电喷雾负离子模式下扫描化合物的分子离子峰、二级质谱碎片信息，并结合保留时间和相关文献，从野生和栽培杨树桑黄子实体中共鉴定出40个化合物，包括小分子有机酸8个、儿茶素类3个、吡喃酮类20个、黄酮9个，其中野生和栽培子实体中共有成分14个，野生子实体中特有成分14个，栽培子实体中特有成分12个。分析结果表明吡喃酮酚类化合物是杨树桑黄子实体中的主要活性成分之一，本研究可为野生和栽培杨树桑黄子实体的快速鉴定、质量控制以及阐明药效物质基础提供参考。


关键词: 杨树桑黄, 吡喃酮, 黄酮, 超高效液相色谱-串联四级杆-飞行时间质谱

Abstract:

 The chemical compositions in wild and cultivated fruiting body of Sanghuangporus vaninii were analyzed rapidly by ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS).Waters Acouity UPLC BEH C18 (100 mm×2.1 mm,1.7 μm) chromatographic column and methanol with water (containing 0.1% formic acid) gradient elution were used to analyze the extracts sample.The ESI-MS negative molecular ion peaks,MS2 product ions,retention time and together with the references were used to tentative identify the chemical constituents.The results indicated that 40 metabolites from wild and cultivated fruiting bodies of S. vaninii were identified,including 8 organic acids,3 catechins,20 pyranones and 9 flavonoids,of which 14 components in wild and cultivated fruiting body,14 unique components in wild and 12 unique components in cultivated fruiting body.The analysis results showed that pyranone phenolic compounds were presumed to be mainly active components in the fruiting body of S. vaninii. This method is rapid,sensitive,which can provide the reference for evaluation of the quality from S. vaninii.

Key words: Sanghuangporus vaninii, pyranone, flavonoid, UPLC-QTOF-MS

中图分类号:  R932 Q946.8

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