天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (6): 1005-1013.doi: 10.16333/j.1001-6880.2022.6.013

• 开发研究 • 上一篇    下一篇

松花粉对环磷酰胺致卵巢早衰大鼠的卵巢保护作用

何毓婕1，曲   涛2，王   桐2，何志勇1，王召君1，秦   昉1，曾茂茂1，陈   洁1*   

  1. 1江南大学 食品科学与技术国家重点实验室，无锡 214122；2烟台新时代健康产业有限公司，烟台 264000
  • 出版日期:2022-06-28 发布日期:2022-06-30
  • 基金资助:
    国家食品科学与技术一流学科项目（JUFSTR20180201）

Protective effect of pine pollen on ovaries of rats with premature ovarian failure induced by cyclophosphamide

HE Yu-jie1,QU Tao2,WANG Tong2,HE Zhi-yong1,WANG Zhao-jun1,QIN Fang1,ZENG Mao-mao1,CHEN Jie1*   

  1. 1State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;2Yantai New Era Health Industry Co.,Ltd.,Yantai 264000,China
  • Online:2022-06-28 Published:2022-06-30

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摘要：

为探讨松花粉对卵巢早衰大鼠卵巢的保护作用及可能机制，采用环磷酰胺（cyclophosphamide，CTX）诱导建立大鼠卵巢早衰模型，研究了不同剂量（1.5、0.5、0.1 g/kg体重）灌胃28天处理下松花粉对卵巢组织病理学、血清激素水平、卵巢的抗氧化能力、抗炎能力和抗颗粒细胞凋亡能力的影响。结果表明，松花粉可提高卵巢指数，减少CTX诱导的闭锁卵泡数量；高剂量松花粉血清激素水平与空白对照组相当；中剂量组显示出与高剂量组相同的抗氧化和抗炎作用，使其恢复至正常水平；松花粉能有效降低颗粒细胞凋亡水平，增加卵巢Bcl-2的表达，降低Bax、Caspase-3和Cleaved Caspase-3的表达。本研究可为开发基于松花粉的卵巢保护功能食品提供理论和实验依据。

关键词: 松花粉, 环磷酰胺, 卵巢早衰, 抗氧化, 抗凋亡, 抗炎

Abstract:

In order to investigate the protective effect and potential mechanisms of pine pollen on the ovaries of rats with premature ovarian failure,cyclophosphamide (CTX) was used to induce premature ovarian failure in rats,and the effects of pine pollen on ovarian histopathology,serum hormone levels,ovarian anti-oxidant capacity,anti-inflammatory capacity,and anti-apoptosis of granulosa cells at different doses (1.5,0.5 and 0.1 g/kg body weight) for 28 days were determined.The results show that pine pollen can increase the ovarian index and reduce the number of atretic follicles induced by CTX.High-dose pine pollen can adjust the serum hormone level to be consistent with that of blank control group.Middle-dose group show similar anti-oxidant and anti-inflammatory effects as high-dose group,bringing the values back to normal levels.Pine pollen can effectively reduce the level of granular cell apoptosis,increase the expression of Bcl-2 in the ovary,and reduce the expression of Bax,Caspase-3,and Cleaved Caspase-3.This study provides a theoretical and experimental basis for the development of functional foods based on pine pollen for ovary protection.

Key words: pine pollen, cyclophosphamide, premature ovarian failure, anti-oxidation, anti-apoptosis, anti-inflammation

中图分类号:  R271.19

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