UPLC characteristic chromatograms of Fructus Citri Immaturus (FCI) and Pericarpium Citri Reticulatae Viride (PCRV) were established,combined with multi-component content determination,so as to provide reference for the quality control of different specifications of PCRV. Waters ACQUITY UPLC HSS T3 (100 mm × 2.1 mm,1.8 μm) column was used. The mobile phase was acetonitrile -0.1% formic acid solution. The flow rate was 0.30 mL per minute with gradient elution. Detection wavelength was 275 nm. The column temperature was 40 °C. The injection volume was 1 μL. The characteristic chromatograms of 15 batches of FCI and PCRV were established. Cluster analysis (HCA),principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to compare the differences between the two specifications of PCRV and look for different components. The contents of five effective components in two specifications of PCRV were compared. The result showed that there were seven common peaks in the characteristic chromatograms of FCI and PCRV. Five common peaks were identified,namely synephrine,narirutin,hesperidin,nobiletin and tangeretin by comparing with the reference substances. HCA and PCA roughly divided PCRV into two categories. three differential markers,namely Peak 1 (synephrine),peak 4 and peak 2 (hesperidin) were found by OPLS-DA. The content determination results showed that the contents of synephrine and hesperidin in Fructus Citri Immaturus were higher than those in PCRV. The contents of nobiletin in PCRV were higher than those in FCI. The differences were statistically significant (P <0.05). This method can effectively analyze the quality differences of different specifications of PCRV and provide reference for the quality evaluation.

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