天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (9): 1562-1568.doi: 10.16333/j.1001-6880.2023.9.010

• 开发研究 • 上一篇    下一篇

油茶叶多酚的提取及其对常见植物病原真菌抑制作用研究

谢水香,雷彩燕*


  

  1. 河南农业大学植物保护学院,郑州 450002
  • 出版日期:2023-09-22 发布日期:2023-09-21
  • 基金资助:
    河南省科技攻关项目(222102110059)

Study on the extraction of polyphenols from Camellia oleifera leaves and its inhibitory effect on common plant pathogenic fungi

XIE Shui-xiang,LEI Cai-yan*   

  1. College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China
  • Online:2023-09-22 Published:2023-09-21

摘要:

目前关于油茶多酚对植物病原真菌抑制作用的报道十分匮乏。本文通过分析不同条件下油茶叶多酚的提取率,确定了最佳的提取条件;分析了不同浓度多酚对常见植物病原真菌的抑制效果并对抑菌机理进行了初步研究。研究结果表明:1∶30的液料比、60%的乙醇浓度、60 min的超声波处理后,多酚提取率最高,达14.01%。每克油茶叶中加入0.80 g β-环糊精,可使多酚提取率提升至22.92%。10%的多酚对新月弯孢霉、假禾谷镰刀菌、链格孢、草莓多主棒孢霉、大丽轮枝菌、葡萄座腔菌、尖孢镰孢菌苦瓜专化型具有一定的抑制作用,对大丽轮枝菌的抑制率高达78.52%。多酚处理对植物病原真菌体内的可溶性蛋白的代谢过程产生了较大的影响,这是植物病原真菌对化学胁迫适应过程的一种自我调节方式。本研究结果为油茶的综合开发以及植物病害的绿色防控提供了理论依据。

关键词: 油茶多酚, 超声提取, 植物病原真菌, 抑菌作用, 可溶性蛋白

Abstract:

At present,there are very few reports on the inhibitory effect of Camellia oleifera polyphenols on plant pathogenic fungi.In this paper,the best extraction method of polyphenols was determined by analyzing the extraction rate of polyphenols in C. oleifera leaves at different conditions,and the inhibitory effect of different concentrations of polyphenols on common plant pathogenic fungi were analyzed and the bacteriostatic mechanism was studied.The results showed that after a liquid to material ratio of 1∶30,ethanol concentration of 60%,and ultrasonic treatment for 60 minutes,the extraction rate of polyphenols was the highest,reaching 14.01%.The addition of 0.80 g β-cyclodextrin in 1.0 g leaves could increase the extraction rate to 22.92%.The polyphenols at concentration of 10% had a certain inhibitory effect on Curvularia lunata,Fusarium pseudograminearum,Alternaria alternata,Corynespora cassiicola,Verticillium dahliae,Botryosphaeria dothidea and Fusarium oxysporum f.sp. momdicae.The inhibition rate to Verticillium dahliae was the highest,which was 78.52%.Polyphenols had great impact on the metabolic process of soluble proteins in plant pathogenic fungic,which is a self-regulation mode of plant pathogenic fungic in adapting to chemical stress.The results of this study provide a theoretical basis for the comprehensive development of C. oleifera and the green prevention and control of plant diseases.

Key words: Camellia oleifera polyphenols, ultrasonic extraction, plant pathogenic fungi, antifungal effect, soluble protein

中图分类号:  R284.2 R965