天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (2): 206-217.doi: 10.16333/j.1001-6880.2024.2.003

• 研究论文 • 上一篇    下一篇

藏药翁布对溃疡性结肠炎的作用机制研究

李新蕊1，陈海娟1,2*，高禹涵1，徐丽1   

  1. 1青海师范大学生命科学学院 青海省青藏高原药用动植物资源重点实验室；2高原科学与可持续发展研究院，西宁810008
  • 出版日期:2024-02-23 发布日期:2024-02-23
  • 基金资助:
    青海省科技厅科技国际合作项目（2021-HZ-805）；青海省“昆仑英才·高端创新创业人才”（2020）；青海师范大学博士科研启动经费（2021）

Study on the mechanism of Tibetan medicine Myricaria germanica on ulcerative colitis

LI Xin-rui1,CHEN Hai-juan1,2*,GAO Yu-han1,XU Li1   

  1. 1Key Laboratory of Medicinal Animal and Plant Resources in Qinghai Tibet Plateau,School of Life Sciences,Qinghai Normal University;2Academy of Plateau Science and Sustainability,Xining 810008,China
  • Online:2024-02-23 Published:2024-02-23

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摘要：

基于网络药理学、分子对接和实验验证，探讨藏药翁布（Myricaria germanica）治疗溃疡性结肠炎（ulcerative colitis，UC）的作用及机制。采用UHPLC-Q-Exactive-Orbitrap-MS技术对翁布原植物化学成分进行快速分析；基于网络药理学预测和筛选翁布治疗UC的核心成分、靶基因和主要通路，并对核心成分和靶点进行分子对接验证；通过葡聚糖硫酸钠（dextran sulfate sodium salt，DSS）诱导UC小鼠模型，观察翁布对UC小鼠的治疗作用。筛选出翁布治疗UC活性成分23种，作用于111个共同靶点；PPI网络分析显示AKT1、TNF、CASP3等可能是核心靶点；GO生物过程主要涉及炎症反应、免疫反应等；KEGG信号通路主要涉及MAPK信号通路、TNF信号通路等；分子对接显示核心靶点TNF、CASP3等与核心活性成分结合良好；翁布可以改善DSS诱导的UC小鼠的结肠长度和结肠黏膜损伤，降低结肠和血清中TNF-α和IL-6的含量。翁布通过大黄酸、异鼠李素、鞣花酸、蟛蜞菊内酯和去甲蟛蜞菊内酯等化合物调节TNF-α、PTGS2等靶点发挥治疗UC的作用。

关键词: 翁布, 溃疡性结肠炎, UHPLC-Q-Exactive-Orbitrap-MS, 网络药理学, 分子对接

Abstract:

Based on network pharmacology,molecular dock and experimental verification,the effect and mechanism of Myricaria germanica in the treatment of ulcerative colitis (UC) were investigated.The UHPLC-Q-Exactive-Orbitrap-MS technique was used for the rapid analysis of the original phytochemical components of M.germanica;the core components,target genes and main pathways of M. germanica for the treatment of UC were predicted and screened based on the network pharmacology,and molecular docking validation was carried out on the core components and targets;and the therapeutic effects of M.germanica on UC were observed by the mouse model of UC induced by dextran sulfate sodium (DSS).Twenty-three active ingredients of M. germanica for the treatment of UC were screened and acted on 111 common targets;PPI network analysis showed that AKT1,TNF,CASP3,etc.might be the core targets;GO biological process mainly involved inflammatory response,immune response,etc.;KEGG signaling pathway mainly involved MAPK signaling pathway,TNF signaling pathway,etc.;molecular docking showed that TNF,CASP3 and other core targets could bind well with the core active components;M. germanica can improve colon length and colonic mucosal damage in DSS-induced UC mice,and reduce the levels of TNF-α and IL-6 in colon and serum. M. germanica exerts its therapeutic effects on UC by modulating TNF-α,PTGS2 and other targets through compounds such as rheic acid,isorhamnetin,ellagic acid,wedelolactone and desmethylwedelolactone.

Key words: Myricaria germanica, ulcerative colitis, UHPLC-Q-Exactive-Orbitrap-MS, network pharmacology, molecular docking

中图分类号:  S859.7

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