不同ω-3/ω-6构成比的配伍红花籽油对SH-SY5Y细胞氧化损伤的预防效应

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 420-424.

不同ω-3/ω-6构成比的配伍红花籽油对SH-SY5Y细胞氧化损伤的预防效应

吴迪1，吴桂荣1，阿布力孜•阿布杜拉2，盛磊2，巴哈尔古丽•卡哈尔1*

1新疆医科大学药学院药化有机教研室，乌鲁木齐 830011； 2新疆医科大学新疆地方病分子生物学重点实验室，乌鲁木齐 830011

收稿日期:2010-06-22 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: bahaerguli_k@126.com
基金资助:
新疆维吾尔自治区重点实验室基金项目（XJDX0208-2006-07）

Preventive Effect of Different ω-3/ω-6 Constituent Ratio of Compatibility Safflower Seed Oil on SH-SY5Y Cells against Oxidative Cell Damage

WU di1, WU Gui-rong1, Abulizi ABUDULA2, SHENG Lei2, Bahaerguli KAHAER1*

1Department of Pharmacochemistry and Organic Chemistry, College of Pharmacy, Medical University of Xinjiang, Urumqi 830011, China; 2Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, College of Preclinical Medicine, Medical University of Xinjiang, Urumqi 830011, China

Received:2010-06-22 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

探讨不同ω-3/ω-6构成比的配伍红花籽油（Compatibility Safflower Seed Oil，CSSO）预防神经细胞氧化损伤的作用。通过过氧化氢（hydrogen peroxide，H₂O₂）氧自由基供体诱导，建立人神经母细胞瘤SH-SY5Y细胞氧化损伤模型；以不同浓度和ω-3/ω-6构成比的CSSO进行细胞药物干预，利用四甲基偶氮唑蓝（methyl thiazolyl tetrazolium，MTT）和流式细胞仪检测细胞活力变化和细胞凋亡率。我们建立了H₂O₂诱导的SH-SY5Y细胞氧化损伤模型，其IC₅₀值为1089.54 μmol/L H₂O₂；随着ω-3相对含量递减，CSSO预防细胞氧化损伤的效应增加，且当ω-3/ω-6比例为1:6.68和有效浓度范围为375~750 μg CSSO/ml时，其药物干预组细胞活力（84.1%）显著高于模型组（61.1%），而药物干预组细胞凋亡率（12.6%）明显低于模型组（25.9%）。从以上结果可以推测，CSSO能够保护细胞并预防氧自由基诱导的细胞损伤，其效果可能与CSSO中ω-3/ω-6构成比密切相关。

关键词: 配伍红花籽油, 氧化损伤, SH-SY5Y, MTT

Abstract:

In order to investigate the preventive effect of different ω-3/ω-6 constituent ratio of compatibility safflower seed oil (CSSO) on SH-SY5Y human neuroblastoma cells against oxidative cell damage, an oxidative cell damage model was established by using hydrogen peroxide (H₂O₂) as a donor of free reactive oxygen species, and after treatment of cells with different ω-3/ω-6 constituent ratios of CSSO, the alteration of cell viability and cellular apoptosis were determined by MTT (methyl thiazolyl tetrazolium) assay and flow cytometry. We successfully established the oxidative cell damage model of SH-SY5Y cells induced by H₂O₂, with the IC₅₀ value of 1089.54 μmol/L H₂O₂. The preventive effect of CSSO against oxidative cell damage increased along with the decreasing amount of ω-3 content in CSSO. Moreover, at the ω-3/ω-6 ratio of 1:6.68 and effective dose range of 375~750 μg/ml CSSO, the cell viability was increased significantly to a higher level in the treatment group (84.1%) than in the control (61.1%), and cellular apoptosis was decreased markedly to a lower level (12.6%) in treatment group than in the control (25.9%). The results suggested that CSSO was able to protect the cells and prevent from oxidative cell damage caused by reactive oxygen species, and this effect may be closely associated with the ω-3/ω-6 constituent ratio in CSSO.

Key words: compatibility safflower seed oil, oxidative damage, SH-SY5Y, MTT

吴迪，吴桂荣，阿布力孜?阿布杜拉，盛磊，巴哈尔古丽?卡哈尔. 不同ω-3/ω-6构成比的配伍红花籽油对SH-SY5Y细胞氧化损伤的预防效应[J]. 天然产物研究与开发, 2011, 23(3): 420-424.

WU di, WU Gui-rong, Abulizi ABUDULA, SHENG Lei, Bahaerguli KAHAER. Preventive Effect of Different ω-3/ω-6 Constituent Ratio of Compatibility Safflower Seed Oil on SH-SY5Y Cells against Oxidative Cell Damage[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 420-424.

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