壳寡糖诱导黄瓜抗黄瓜黑星病的初步研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 508-511.

壳寡糖诱导黄瓜抗黄瓜黑星病的初步研究

解婷婷，吕淑霞*，马镝，张良，王媛媛

沈阳农业大学生物科学技术学院，沈阳 110866

收稿日期:2010-11-29 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: lushuxia@hotmail.com

Primary Study on the Resistance to Cladosporium cueumerinum of Cucumber Induced by Chito-oligosaccharide

XIE Ting-ting,LV Shu-xia*,MA Di,ZHANG Liang,WANG Yuan-yuan

College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,China

Received:2010-11-29 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

本文研究了壳寡糖诱导黄瓜对黑星病的抗性作用。利用6 mg/mL壳寡糖溶液对苗期黄瓜诱导，进行病情调查统计及测定处理前后黄瓜叶片的主要防御酶系——苯丙氨酸解氨酶，过氧化物酶，多酚氧化酶，超氧化物歧化酶，过氧化氢酶的活性变化。结果显示，壳寡糖对黄瓜黑星病在10 d和17 d的诱抗效果分别为60.25%和47.59%，且作为诱导因子可显著提高黄瓜叶片内苯丙氨酸解氨酶（PAL）活性，过氧化物酶（POD）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD）活性也有所提高，但叶片内过氧化氢酶（CAT）活性无较明显变化。研究结果表明壳寡糖对黄瓜抗黑星病产生诱导作用，为研究壳寡糖作为新型生物农药提供了依据。

关键词: 壳寡糖, 黄瓜, 黄瓜黑星病, 诱导抗性, 防御酶系

Abstract:

This article focused on the cucumber resistance to Cladosporium cueumerinum which was induced by chito-oligosaccharide.The state of illness of the cucumber seedlings induced by 6mg/mL chito-oligosaccharide was investigated and the activities of major defense enzymes of phenylalanin ammo-nialyase (PAL),peroxidase (POD),polyphenol oxidase (PPO),superoxide dismutase (SOD),and catalase (CAT) were determined.The results showed that the induced resistance of cucumber scab by chito-oligosaccharide was 60.25% and 47.59% in 10 d and 17 d,respectively.The activity of PAL increased obviously and POD,PPO,SOD also increased partly,but chito-oligosaccharide had no effect on CAT.These researches suggested that resistance to cucumber C.cueumerinum induced by chito-oligosaccharide would be helpful in using chito-oligosaccharide as a new biological pesticide.

Key words: chito-oligosaccharide, cucumber, Cladosporium cueumerinum, induced resistance, defense enzymatic system

中图分类号:

Q936

解婷婷，吕淑霞，马镝，张良，王媛媛. 壳寡糖诱导黄瓜抗黄瓜黑星病的初步研究[J]. 天然产物研究与开发, 2011, 23(3): 508-511.

XIE Ting-ting,LV Shu-xia,MA Di,ZHANG Liang,WANG Yuan-yuan. Primary Study on the Resistance to Cladosporium cueumerinum of Cucumber Induced by Chito-oligosaccharide[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 508-511.

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