同时提取光果甘草中光甘草定和甘草酸的工艺研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 547-550.

同时提取光果甘草中光甘草定和甘草酸的工艺研究

李玉会，徐德平，张连富*

江南大学食品学院，无锡 214122

收稿日期:2010-05-13 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: zhanglianfu@hotmail.com
基金资助:
科技部“十一五”国家科技支撑计划（2006BAD27B03）

Simultaneous Extraction of Glabridin and Glycyrrhizic Acid from Glycyrrhiza glabra L.

LI Yu-hui, XU De-ping, ZHANG Lian-fu*

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2010-05-13 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

本文主要对光果甘草中光甘草定、甘草酸两种重要活性成分的同时提取工艺进行了研究。通过单因素试验确定了影响提取的主要因素及适宜水平范围，通过正交试验确定了其最佳提取条件为：用含0.6%氨水的60%乙醇溶液作为提取剂，料液比为1:20（w:v），在75 ℃条件下提取2 h。最佳提取条件下光甘草定的得率为0.238±0.002%，甘草酸的得率为5.08±0.03%。该工艺操作简单，投入一批原料，即可同时提取其中的光甘草定和甘草酸，为实现甘草资源高附加值的综合加工利用奠定了基础。

关键词: 光果甘草, 光甘草定, 甘草酸, 提取

Abstract:

To study the technology of simultaneous extraction of glabridin and glycyrrhizic acid from Glycyrrhiza glabra L., the single factor experiments were done to determine the influencing factors and the orthogonal tests were done to optimize the extracting condition. The extraction conditions were optimized as follows: 60% ethanol containing 0.6% ammonia as extractant, ratio of material to liquid 1:20 (w:v), extraction temperature 75 ℃, and time 2 h. Under these conditions, the extraction rates of Glabridin and Glycyrrhizic acid were 0.238±0.002% and 5.08±0.03% respectively. This technology featured simple operation and yielded the two important bioactive compounds at the same time. The research provided a basis for comprehensive development and utilization of Glycyrrhiza resources with high valued-added.

Key words: Glycyrrhiza glabra L., glabridin, glycyrrhizic acid, extraction

中图分类号:

TS202.3 R282.4 R284.2

李玉会，徐德平，张连富. 同时提取光果甘草中光甘草定和甘草酸的工艺研究[J]. 天然产物研究与开发, 2011, 23(3): 547-550.

LI Yu-hui, XU De-ping, ZHANG Lian-fu. Simultaneous Extraction of Glabridin and Glycyrrhizic Acid from Glycyrrhiza glabra L.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 547-550.

参考文献 12

1	Bai FM(白凤梅). Function and main properties of Glycyrhizin.Global Food Ind(中外食品),2003, (1):13.
2	Wang ZL(王照兰),Du JC(杜建材),Yu LQ(于林清),et al.The utilization value, current research and problems of Glycyrrhiza.Grassland China(中国草地),2002,24(1):73-75.
3	Vaya J,Belinky PA,Aviram M.Antioxidant constituents from licorice roots: isolation structure elucidation and antoxidative capacity toward LDL oxidation.Free Radic Biol Med,1997,23:302-313.
4	Fuhrman B,Vol kova N,Kaplan M,et al.Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients: increased resistance of LDL to atherogenic modifications, reduced plasma lipid levels,and decreased systolic blood pressure.Nutrition,2002,18:268-273.
5	Mira R,Paula B,Jacob V,et al.Macrophage enrichment with the isoflavan glabridin inhibits NADPH oxidase-induced cell-mediated oxidation of low density lipoprotein.J Biol Chem,1999,274:13790-13799.
6	Aoki F,Nakagawa K,Kitano M,et al.Clinical safety of licorice flavonoid oil (LFO) and pharmacokinetics of glabridin in healthy humans.J Am Coll Nutr,2007,26(3):209-218.
7	Fuhrman B,Bush S,Vaya J,et al.Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation:in vitro and ex vivo studies in humans and in atherosclerotic apolipoprotein E-deficient mice.Am J Clin Nutr,1997,66:267-275.
8	Okada K,Tamura Y,Yamamoto M,et al.Identification of antimicrobial and antioxidant constituents from licorice of Russian and Xinjiang origin.Chem Pharm Bull,1989,37:2528-2530.
9	Mourboul A(木合布力·阿布力孜), Rena K(热娜·卡斯木), Ma SY(马淑燕),et al.Isolation and antioxidant property of glabridin.Nat Prod Res Dev(天然产物研究与开发),2007,19:675-682.
10	Shanker K,Fatima A,Negi AS,et al.RP-HPLC method for the quantitation of glabridin in Yashti-madhu (Glycyrrhiza glabra).Chromatographia,2007, 65:771-774.
11	Li J(李佳),Song XB(宋新波),Yu BL(余保林),et al.Content determination of glabridin in G. glabra L. with HPLC. Tianjin J Tradit Chin Med(天津中医药), 2008, 25(2):157-158.
12	Chinese Pharmacopoeia Commission(国家药典委员会).Pharmacopoeia of the People's Republic of China (中国药典), Vol. I. Beijing: Chemical Industry Press, 2005.59-60.

[1]	代千千, 胡娜, 王洪伦, 丁晨旭. 中国被毛孢亚临界水提取多糖结构特征及抗氧化活性[J]. 天然产物研究与开发, 2023, 35(增刊2): 46-54.
[2]	陈静华, 吴小可, 祁萍喜, 于瑞涛. 唐古特铁线莲石油醚提取物的GC-MS分析[J]. 天然产物研究与开发, 2023, 35(增刊2): 72-75.
[3]	丁林玲, 胡江苗, 刘洪均, 包誉, 李晓珏, 华燕. 超声辅助酶法提取漆蜡工艺优化和理化性质研究[J]. 天然产物研究与开发, 2023, 35(增刊2): 84-92.
[4]	李欣聪, 宋学英, 施子涵, 王彩芳. 金银花不同提取方法的比较研究[J]. 天然产物研究与开发, 2023, 35(增刊2): 99-103.
[5]	刘婷婷. 水飞蓟宾的植物来源、提取方法和抗癌活性研究进展[J]. 天然产物研究与开发, 2023, 35(增刊2): 154-163.
[6]	丁金, 铁芳芳, 胡娜, 王洪伦, 董琦. 响应面优化提取山莨菪生物碱的工艺及其抗炎活性研究[J]. 天然产物研究与开发, 2023, 35(增刊1): 73-79.
[7]	李彩霞, 多杰措, 宋文珠, 刘凤香, 马世震. 洋葱精油提取方法、化学成分及生物活性研究进展[J]. 天然产物研究与开发, 2023, 35(增刊1): 153-158.
[8]	江程, 方晒, 李远文, 吴伟东, 陈瑞云, 顾琼. 臭草二氯甲烷部位化学成分研究[J]. 天然产物研究与开发, 2023, 35(9): 1518-1527.
[9]	谢水香, 雷彩燕. 油茶叶多酚的提取及其对常见植物病原真菌抑制作用研究[J]. 天然产物研究与开发, 2023, 35(9): 1562-1568.
[10]	蒋蜜涵, 廖灵洁, 廖阳, 黄卓颖, 王丽倩, 于凌一丹, 闫荣玲. 银杏外种皮与垂序商陆叶提取物复配剂对小菜蛾及十字花科作物幼苗的生理影响[J]. 天然产物研究与开发, 2023, 35(9): 1583-1590.
[11]	刘巨钊, 鲜梦雪, 孔伟华, 田晓黎, 袁强, 崔琦. 草果精油提取工艺优化与成分分析[J]. 天然产物研究与开发, 2023, 35(5): 766-780.
[12]	高倩玲, 胡龄文, 陈鸿平, 刘友平, 陈林, 胡媛. 枸杞过氧化物酶的提取工艺优化及其酶学特性研究[J]. 天然产物研究与开发, 2023, 35(5): 819-827.
[13]	张俊焱, 李成慧, 左文明, 李锦萍, 刘力宽, 曾阳. 金露梅中一种α-葡萄糖苷酶抑制剂的提取富集及活性研究[J]. 天然产物研究与开发, 2023, 35(3): 460-466.
[14]	张宽朝, 徐晨阳, 余佳琪, 苏鸿呈, 徐忠有. 牡蒿总黄酮的提取、纯化及抗氧化活性分析[J]. 天然产物研究与开发, 2023, 35(2): 281-290.
[15]	谭武宾, 周松, 康海, 蒋湘勇, 毛正, 杨科, 李铁求. 基于网络药理学和实验验证探讨光甘草定治疗去势抵抗性前列腺癌的作用机制[J]. 天然产物研究与开发, 2023, 35(2): 310-318.