高效液相法与硫酸-蒽酮法测定猪苓多糖含量比较

doi:A

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (6): 1099-1102.doi: A

高效液相法与硫酸-蒽酮法测定猪苓多糖含量比较

张国伟¹，李彩霞²，王艳峰³，黄羽²，苏芝军⁴，郑芳²，曾星^2*

¹河北大学中医学院，保定 071002；²广州中医药大学第二临床医学院，广州 510006； ³中国医药集团四川抗菌素工业研究所，成都 610051；⁴陕西省府谷县人民医院，府谷 719400

收稿日期:2010-08-24 出版日期:2011-12-22 发布日期:2012-07-04
基金资助:
国家自然科学基金（30873426）；广东省科技计划项目（2010B030700059）；河北大学科研基金资助项目(2010Q46)

Determination and Comparison of Polyporus Polysaccharide by HPLC and Anthrone-Sulfuric Acid Method

ZHANG Guo-wei¹, LI Cai-xia², WANG Yan-feng³, HUANG Yu², SU Zhi-jun⁴, ZHENG Fang², ZENG Xing^2*

¹College of Chinese Medicine, Hebei University, Baoding 071002, China; ²Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; ³Sinopharm ChuanKong pharmaceutical CO., LTD, Chengdu 610051, China;⁴Fugu Peoples Hospital of Shanxi, Fugu 719400, China

Received:2010-08-24 Online:2011-12-22 Published:2012-07-04

摘要/Abstract

摘要： 本研究目的是比较高效液相法与硫酸-蒽酮法在测定猪苓多糖含量上的差异。为此，研究采用水提醇沉、Sevag法除蛋白、透析和冷冻干燥制备相对纯化的猪苓多糖，通过凝胶渗透色谱及高效液相法对猪苓多糖的相对分子量分布、组成及含量进行研究，用硫酸-蒽酮分光光度法测定猪苓颗粒中猪苓多糖的含量。结果显示，猪苓多糖数均相对分子质量为48,232，重均相对分子质量为117,506，分子范围2.44，可能由海藻糖和葡萄糖组成，其含量分别为6.05%和62.28%。硫酸-蒽酮法侧的猪苓多糖含量为87.9%。对于猪苓多糖含量测定，高效液相法优于硫酸-蒽酮法。

关键词: 猪苓多糖, 高效液相法, 硫酸-蒽酮法

Abstract: This method is aimed to compare the differences between HPLC and anthrone-sulfuric acid method in determining Polyporus Polysaccharide (PPS).PPS was prepared by water extracting-alcohol precipitating, deproteinization with Sevag method, dialysis and freeze-drying. Relative molecular weight of PPS was determined by Gel Permeation in Chromatography (GPC) and the constitutes were analyzed by HPLC. At the same time, anthrone-sulfuric acid method was used to measure the content and purity of PPS.The results showed that the number-average molecular weight (Mn) of PPS wass 48,232, weight-average molecular weight (Mw) was 117,506, the polydispersity (Mw/Mn) was 2.44. PPS was composed of glucose (62.28%) and trehalose (6.05%). The PPS purify degree was 87.9% by anthrone-sulfuric acid method. Conclusively, HPLC method was superior to anthrone-sulfuric acid method in determining of PPS content.

Key words: Polyporus polysaccharide, HPLC, anthrone-sulfuric acid method

中图分类号:

R284.2

张国伟，李彩霞，王艳峰，黄羽，苏芝军，郑芳，曾星. 高效液相法与硫酸-蒽酮法测定猪苓多糖含量比较[J]. 天然产物研究与开发, 2011, 23(6): 1099-1102.

ZHANG Guo-wei, LI Cai-xia, WANG Yan-feng, HUANG Yu, SU Zhi-jun, ZHENG Fang, ZENG Xing. Determination and Comparison of Polyporus Polysaccharide by HPLC and Anthrone-Sulfuric Acid Method[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(6): 1099-1102.

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高效液相法与硫酸-蒽酮法测定猪苓多糖含量比较

Determination and Comparison of Polyporus Polysaccharide by HPLC and Anthrone-Sulfuric Acid Method

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