具杀螺活性的商陆根际真菌Aspergillus fumigatus SL-30发酵条件优化

doi:A

摘要/Abstract

摘要： 以杀螺率为响应值，对商陆根际真菌菌株SL-30的发酵条件进行优化。在前期单因素试验的基础上，采用Plackett-Burman设计法，对影响菌株发酵滤液杀螺活性的主要因素进行筛选，确定了三个主要因素依次为可溶性淀粉，蛋白胨和初始pH。采用响应面分析法确定了以上三个显著因子的最佳水平分别及交互影响，结果显示，三个显著因素的最佳水平为可溶性淀粉16.40 g/L，蛋白胨3.76 g/L，初始pH 3，该条件下发酵滤液的杀螺活性预测值达98.949%。经六批次的发酵验证，预测值与试验平均值接近。

关键词: 杀螺活性, 根际真菌, 发酵条件, 响应面分析

Abstract: Fermentation conditions of a rhizospheric strain named as SL-30 from Phytolacca acinosa Roxb. were optimized. On the basis of early study, Plackett-Burman design was undertaken to evaluate the effects of the eight factors and three statistically significant parameters including soluble starch, peptone and initial pH were selected. Then a response surface methodology was used to optimize the above three factors, and to find out the optimal concentration levels and the relationships between each pair of these factors. The result showed, under conditions of soluble starch 16.40 g/L, peptone 3.76 g/L and initial pH 3 the molluscicidal activity of fermentation broth’s filtrate reached 98.949%. After six batches cultivation, the predicted values were verified by validation experiments.

中图分类号:

Q815

郭丹钊，陈钧，杜向萍，张春晓，潘静. 具杀螺活性的商陆根际真菌Aspergillus fumigatus SL-30发酵条件优化[J]. 天然产物研究与开发, 2012, 23(09): 1176-.

GUO Dan-zhao, CHEN Jun, DU Xiang-ping, ZHANG Chun-xiao, PAN Jing. Optimization of Fermentation Conditions for Molluscicidal Rhizosphere Fungi Aspergillus fumigatus from Phytolacca acinosa Roxb.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1176-.

参考文献

1 Zhou XN,Wang LY,Chen MG,et al.The public health significance and control of Schistosomiasis in China-then and now. Acta Trop. 2005, 96(2-3): 97-105.
2 dos Santos AF, de Azevedo DP,dos Santos Mata Rda C,et al. The lethality of Euphorbia conspicuato adults of Biomphalaria glabrata，cercaria of Schistosoma mansoni and larvae of Artemia salina. Bioresour Technol,2007,98:135-139.
3 Andrews P, Thyssen J, Lorke D. The biology and toxicology of molluscicide, Bayluscide. Pharmacol Ther, 1982,19(2):245-295.
4 Mao ZH(毛佐华),Yu PZ(俞培忠),Sun K(孙锴),et al.Preparation of five ginkgolic acid monomers and their molluscicidal effects against Oncomelania hupensis.Chin J Parasitol Parasit Dis(中国寄生虫学与寄生虫病杂志),2007,25:274-278.
5 Yang JM(杨建明),Xiao RF(肖瑞芬),Zhou Y (周艳). Studies on killing snail of microorganism.J Hubei Univ (Nat Sci) (湖北大学学报（自然科学版）),2003,25:337-341.
6 Guo DZ,Chen J,Du XP,et al.Screening of molluscicidal strain against Oncomelania hupensis from rhizosphere of medicinal plant Phytolacca acinosa Roxb.Phcog Mag,2010,6:159-165.
7 Wang WL(王维乐),Mu ZM(牟志美),Zhang SJ(张淑君),et al.Optimization of Fermentation Conditions of Paraconiothyrium variabile GHJ-4 for Laccase Production by Response Surface Methodology.Chin J Appl Environ Biol (应用与环境生物学报), 2011, 17:321-325.
8 Singh R,Kuma R,Bishnoi K,et al.Optimization of synergistic parameters for themostable cellulase activity of Aspergillus heteromorphus using response surface methodology.Biochem Eng J,2009,48(1):28-35.
9 Shentu XP(申屠旭萍),Shi YJ(石一珺),Yu XP(俞晓平).Optimization of Conditions for Production of Trichodermin by Trichoderma harzianum. Chin J Biolog Cont (中国生物防治),2009,25:348-354.
10 Chen Y(陈燕),Wang WP(王文平),Qiu SY(邱树毅),et al.Optimization of Ultrasonic-assisted Extraction of Coix lacryma-jobi Seed Ester Using Response Surface Methodology.Food Sci(食品科学),2010,31(8):46-50.
11 Ge JP(葛菁萍),Liu GM(刘国明),Sun HB(孙红兵),et al.Response Surface Methodology to Optimize the Hydrolysis Condition of Corncob Hemicellulose.Chin Agric Sci Bull (中国农学通报),2011,27(18):64-64.
12 He H(何欢),Wang ZW (王占武),Hu D(胡栋),et al.Progress on Interactions between Root Exudates and RhizosphereM icroorganisms. J Hebei Agric Sci(河北农业科学),2011,15(3):69-73.
13 Zhou GY(周国英),Gou ZH(苟志辉),Hao Y(郝艳),et al.Screening antagonistic silicate bacteria from Camellia oleifera rhizosphere against Fusarium proliferatum and stability of bacteria.J Cent South Univ For Tech(中南林业科技大学学报),2010,30:118-122.
14 He RF (何仁发),Li MY (李明月),Wang J(王娟),et al.Aspergillus sp. F5 from the rhizospheric soil of Bruguiera gymnorrhiza and its antibacterial metabolites. Mycosystema (菌物学报),2010,29:732-738.
15 Xiao TJ(肖同建),Chen F(陈芳),Zhu Z(朱震),et al.Effect of different rhizobacteria on suppression root-knot nematode (Meloidogyne incognita) of tomato.J Nanjing Agric Univ (南京农业大学学报),2011,34(4):59-64.

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