巴西蜂胶对磷脂酰胆碱特异性磷脂酶C活性的影响

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (1): 128-130.

巴西蜂胶对磷脂酰胆碱特异性磷脂酶C活性的影响

玄红专1,2，李振2，张丽2，宋玉冬2，胡福良1*

1 浙江大学动物科学学院，杭州 310029；2 聊城大学生命科学学院，聊城 252059

出版日期:2014-01-31 发布日期:2014-12-15

Effect of Brazilian Propolis on the Activity of Phosphatidylcholine-specific Phospholipase C

XUAN Hong-zhuan1,2, LI Zhen2, ZHANG Li2, SONG Yu-dong2, HU Fu-liang1*

1 College of Animal Sciences,Zhejiang University,Hangzhou 310029,China; 2 School of Life Science,Liaocheng University,Liaocheng 252059,China

Online:2014-01-31 Published:2014-12-15

摘要/Abstract

摘要： 去除血清和生长因子条件下研究巴西蜂胶对磷脂酰胆碱特异性磷脂酶C（PC-PLC）活性的影响。去除血清和生长因子诱导内皮细胞（VECs）凋亡，经12.5、25和50 μg/mL的巴西蜂胶处理VECs 24 h，MTT法检测细胞的存活率；以L-α-卵磷脂为底物测定PC-PLC的活性；免疫细胞化学法检测PC-PLC的表达。结果表明高浓度的巴西蜂胶降低VECs存活率，抑制PC-PLC的活性及表达。巴西蜂胶对VECs的影响具有剂量依赖性，今后巴西蜂胶应用中应考虑剂量对内皮细胞的影响。

关键词: 巴西蜂胶, 磷脂酰胆碱特异性磷脂酶C, 存活率

Abstract: To understand the effects of Brazilian propolis on the activity and expression of phosphatidylcholine-specific phospholipase C (PC-PLC) in vascular endothelial cells (VECs) deprived of serum and basic fibroblast growth factor (FGF-2).Confluent cells were deprived of serum and FGF-2,then the cells were divided for treatment by Brazilian propolis 12.5,25 and 50 μg/mL for 24 h.Cell viability was measured by MTT method,the activity of PC-PLC was determined by using L-α-phosphatidylcholine as the substrate,the expression of PC-PLC was tested by immunohistochemistry.Results showed that treatment of Brazilian propolis 25 and 50 μg/mL for 24 h significantly depressed VECs viability,and the activity and expression of PC-PLC were also decreased by high concentration Brazilian propolis.The effects of Brazilian propolis on VECs had dose-dependent,and considering the safety of using Brazilian propolis,more attention should be paid on the concentration of propolis.

Key words: Brazilian propolis, phosphatidylcholine-specific phospholipase C, cell viability

中图分类号:

R363

玄红专1,2，李振2，张丽2，宋玉冬2，胡福良1*. 巴西蜂胶对磷脂酰胆碱特异性磷脂酶C活性的影响[J]. 天然产物研究与开发, 2014, 26(1): 128-130.

XUAN Hong-zhuan1,2, LI Zhen2, ZHANG Li2, SONG Yu-dong2, HU Fu-liang1*. Effect of Brazilian Propolis on the Activity of Phosphatidylcholine-specific Phospholipase C[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(1): 128-130.