天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 133-138.

• 开发研究 • 上一篇    下一篇

竹叶总黄酮提取工艺的比较研究

叶清华,林晓燕,张舒婷,周相助,陈清西*   

  1. 福建农林大学园艺学院,福州 350002
  • 出版日期:2014-12-30 发布日期:2015-03-04

Comparative Research on Extraction of Total Flavonoids from Bamboo Leaves

YE Qing-hua,LIN Xiao-yan,ZHANG Shu-ting,ZHOU Xiang-zhu, CHEN Qing-xi*   

  1. School of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 本试验采用水浴法、超声法提取箬竹叶总黄酮,通过单因素和正交试验,获得两种提取方法的最优参数。结果表明:水浴法提取竹叶总黄酮的最佳工艺参数为:乙醇浓度90%、液固比25:1(mL/g)、提取时间0.5 h、提取温度为70 ℃,提取3次,提取得率为1.79%。超声法提取竹叶总黄酮的最佳工艺参数为:乙醇浓度90%、液固比35:1 (mL/g)、提取时间75 min、提取功率360 W,提取2次,提取得率为2.75%。超声法优于水浴法。

关键词: 竹叶, 总黄酮, 提取, 超声, 水浴, 优化

Abstract: This experiment explored water bath extraction and supersonic to extract the content of total flavonoids from bamboo leaves.Via single factor experiment and orthogonal test,two methods optimal extract parameters were acquired.The results showed the optimal parameters for the extraction of total flavones from bamboo leaves by water bath method:Ethanol concentration 90%,liquidsolid ratio of 25:1(mL/g),extraction time 0.5 h,extraction temperature(water bath)70 ℃,for 3 times,the extracting rate was 1.79%.The optimal parameters for the extraction of total flavones from bamboo leaves by supersonic:Ethanol concentration 90%,liquidsolid ratio of 35:1(mL/g),extraction time 75 min,extraction power 360 W,for twice,the extracting rate was 2.75%.The method of supersonic was better than water bath.

Key words: Bamboo leaves, Total flavonoids, Extraction, Water bath, Supersonic, Optimization

中图分类号: 

Q946 R284.2