天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (3): 453-463.doi: 10.16333/j.1001-6880.2020.3.015

• 研究简报 • 上一篇    下一篇

来源于大兴安岭多年冻土可培养真菌及其发酵物的生物活性

李泽宇,张志,邱天艺,刘振伟,张哲栋,高思禹,徐利剑*   

  1. 黑龙江大学现代农业与生态环境学院,哈尔滨 150080
  • 出版日期:2020-03-28 发布日期:2020-05-12
  • 基金资助:

    国家自然科学基金(31870528);黑龙江省博士后科研启动金(LBH-Q15123);黑龙江大学创新训练省级指导项目(201910212175)

Culturable fungi from permafrost of Greater Khingan Mountains and biological activities of their fermented products

LI Ze-yu,ZHANG Zhi,QIU Tian-yi,LIU Zhen-wei,ZHANG Zhe-dong,GAO Si-yu,XU Li-jian*   

  1. College of Agricultural Resource and Environment of Heilongjiang University,Harbin 150080,China
  • Online:2020-03-28 Published:2020-05-12

摘要: 本研究目的是分离大兴安岭多年冻土中的可培养真菌并测试它们产生的天然产物的生物活性。首先利用颗粒法在冻土中得到66株可培养真菌,通过内部转录间隔区序列对它们进行分子鉴定,发现它们隶属于55个分类单元,有8株真菌的ITS相似性≤97%。其中5株真菌属于耐冷真菌。研究结果发现6株真菌的提取物具有抗菌活性,其中外担子菌FSF028号菌株活性最强,同时具有抗真菌与抗细菌活性;柄孢壳菌FSF042号菌株具有较好的抗氧化活性;镰刀菌FSF029号菌株有较好的降解纤维素能力;此外还首次发现了Seltsamia属真菌具有抗氧化与产纤维素酶活性。

关键词: 多年冻土, 可培养真菌, 大兴安岭, 次级代谢物, 抗菌活性

Abstract: Sixty-six culturable fungi were isolated from the permafrost in Greater Khingan Mountains by particle isolation method and their natural products were screened for the biological activities.These fungi belonged to 55 taxa using the sequences of Internal Transcribed Spacer (ITS) and of them,the ITS similarities of 8 fungal strains were less than 97%.In those 8 fungal strains,six fungal strains exhibited antimicrobial activities and of them,Exobasidium sp. FSF028 was the strongest one.Podospora sp. FSF042 exhibited the strongest antioxidant activity and Fusarium sp. FSF029 exhibited a significant producing cellulase activity.In addition,this is the also first report that Seltsamia had antioxidant and producing cellulase activities.

Key words: permafrost, culturable fungus, Greater Khingan Mountains, secondary metabolite, antimicrobial activity

中图分类号: 

Q939.96