天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (12): 2016-2023.doi: 10.16333/j.1001-6880.2024.12.003 cstr: 32307.14.1001-6880.2024.12.003

• 研究论文 • 上一篇    下一篇

琥珀酸钠对高脂饮食小鼠代谢和肠道菌群的调节作用

宋彪章1,2，曹宏1,2,3,4，赵丽彤5，张烽1,2,3,4，闫继爱1,3,4，马雪莹6，李   丹1,3,4*，杨   菊1,3,4*   

  1. 1江南大学附属医院临床营养科；2江南大学生物工程学院；3江南大学医学院，无锡 214000；4江苏集萃未来食品技术研究所有限公司，宜兴 214200；5宁波市第二医院，宁波 315000；6无锡市食品安全检验检测中心，无锡 214000
  • 出版日期:2024-12-26 发布日期:2024-12-26
  • 基金资助:
    江南大学附属医院研究型医院医学科研项目（YJZ202305）；国家市场监管技术创新中心（特殊食品）2023年开放课题（TIS F2023001）；国家自然科学基金青年科学基金（32101033）；江苏省自然科学基金青年科学基金（BK20210060）

Regulatory effects of sodium succinate on metabolism and intestinal flora in mice fed a high-fat diet

SONG Biao-zhang1,2,CAO Hong1,2,3,4,ZHAO Li-tong5,ZHANG Feng1,2,3,4,YAN Ji-ai1,3,4,MA Xue-ying,LI Dan1,3,4*,YANG Ju1,3,4*
  

  1. 1Clinical Nutrition Department,Affiliated Hospital of Jiangnan University,Wuxi 214000,China;2School of Biotechnology,
    Jiang Nan University,Wuxi 214000,China; 3Medical School,Jiang Nan University,Wuxi 214000,China;4Jiangsu Jicui Future Food Technology Research Institute Co.,Ltd.,Yixing 214200,China;5Ningbo NO.2 Hospital,Ningbo 315000,China;6Wuxi Food Safety Inspection and Testing Center,Wuxi 214000,China

  • Online:2024-12-26 Published:2024-12-26

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摘要：

研究不同剂量琥珀酸钠（sodium succinate，SS）对高脂饮食小鼠代谢指标及肠道菌群的改善作用。将32只6周龄C57BL/6J小鼠高脂饮食8周诱导小鼠肥胖模型后，随机分为4组，即高脂饮食组、低剂量、中剂量、高剂量琥珀酸钠组。通过定期记录体重，代谢实验、检测血清血脂四项和促炎细胞因子水平、组织学分析、16S rRNA基因测序等方法来研究琥珀酸钠对高脂饮食小鼠代谢和肠道菌群的影响。结果显示，琥珀酸钠可以减缓高脂饮食小鼠体重的增加，改善血脂和炎症水平、减少附睾白色脂肪细胞的扩张以及棕色脂肪白色化，并且调节了肠道菌群的结构和组成。本研究表明琥珀酸钠通过提高高脂饮食小鼠的呼吸代谢作用增加能量消耗，提高Akkermansia、Peptostreptococcaceae、Allobaculum的相对丰度，降低Lachnospiraceae、Ileibacterium、Desulfovibrionaceae的相对丰度来改善高脂饮食小鼠的代谢紊乱和体重增加。

关键词: 琥珀酸钠, 高脂饮食, 呼吸, 促炎细胞因子, 肠道菌群

Abstract:

This study aims to investigate the effects of different doses of sodium succinate on metabolic indicators and intestinal flora in mice on a high-fat diet.After inducing obesity in 32 six-week-old C57BL/6J mice with an eight-week high-fat diet,the mice were randomly divided into four groups:a high-fat diet group,and a low-dose,a medium-dose,and a high-dose sodium succinate group.Various methods were carried out to study the effects of sodium succinate on the metabolism and intestinal flora of mice fed a high-fat diet,including regular weight recording,metabolic cage experiments,serum lipid and proinflammatory cytokine level measurements,histological analysis,and 16S rRNA gene sequencing.The results indicate that sodium succinate could slow down the weight gain,decrease blood lipid and inflammation levels,reduce the expansion of epididymal adipose white tissue and the whitening of brown adipose tissue,and regulate the structure and composition of intestinal flora in HFD-fed mice.This study indicates that sodium succinate decreased the metabolic disorders and weight gain in mice fed a high-fat diet by increasing energy expenditure through enhanced respiratory metabolism.It also increased the relative abundance of Akkermansia,Peptostreptococcaceae,and Allobaculum,while decreasing the relative abundance of Lachnospiraceae,Ileibacterium,and Desulfovibrionaceae.

Key words: sodium succinate, high fat diet, breathe, proinflammatory cytokine, intestinal flora

中图分类号:  R259.2

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