天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (4): 498-504.doi: 10.16333/j.1001-6880.2016.4.006

• 研究论文 • 上一篇    下一篇

采用滤袋技术快速测定杜仲叶片中杜仲胶含量

刘慧东1,2,马志刚1,2,3,朱景乐1,2,3,伊焕4,杜红岩1,2,3,孙志强1,2,3*   

  1. 1 国家林业局泡桐研究开发中心,郑州 450003;2 中国林科院经济林研究开发中心,郑州 450003;3 国家林业局杜仲工程技术研究中心,郑州 450003;4 商丘市梁园区林业局,商丘 476000
  • 出版日期:2016-04-30 发布日期:2016-05-13

Fast Determination of Eucommia Rubber Content in Eucommia ulmoides Leaves Using Filter Bag Technology

LIU Hui-dong1,2,MA Zhi-gang1,2,3,ZHU Jing-le1,2,3,YI Huan4,DU Hong-yan1,2,3,SUN Zhi-qiang1,2,3*   

  1. 1 Paulownia R&D Center of China,Chinese Academy of Forestry CAF,Henan Zhengzhou 450003,China; 2 Non-timber Forest R&D Center,CAF,Henan Zhengzhou 450003,China; 3 Eucommia  Engineering Research Center of State Forestry Administration,Henan Zhengzhou 450003,China;  4 Forestry Bureau of Liangyuan District of Shangqiu,Henan Shangqiu 476000,China
  • Online:2016-04-30 Published:2016-05-13

摘要: 采用滤袋技术依据减重法原理测定了杜仲叶片中杜仲胶含量。通过正交设计比较了不同滤袋孔径、样品量和提取时间对提取效果的影响,并采用该方法比较了同一产地不同品种、同一品种不同产地的杜仲叶中胶含量的差异。结果表明,不同孔径的提取效果差异显著(F=3.685,p=0.043),样品量和提取时间对提取效果无显著影响。适宜的测定条件是样品量0.1 g、滤袋孔径25 μm/10 μm、提取时间60 min。滤袋法测得的‘华仲6号’胶含量为4.49%,传统碱煮法为3.64%。同一产地不同品种、同一品种不同产地的杜仲叶中胶含量差异极显著(F=92.689,P=0.001),其中郑州的‘华仲11号’含量最高,平均为7.00%,灵宝的‘华仲12号’含量最低,为4.28%。生产杜仲胶的原料应标明品种、产地以及树龄,这样有助于合理评估预期产量和生产成本。

关键词: 杜仲, 杜仲胶含量, 提取率, 减重法, 滤袋技术

Abstract: Based on weight lost method,filter bag technology (FBT) was applied to determine rubber content in Eucommia ulmoides leaves.An orthogonal design was applied to compare the effect of filter bag aperture,sample weight and extraction time on extraction efficacy.The rubber content in leaves from different clones and the same clone planted in different sites were then compared using FBT.Results indicated that rubber content varied significantly among different filter bag apertures (F=3.685,p=0.043),while it was not impacted significantly by sample weight and extraction time.The optimal condition for determining rubber content was 25 μm or 10 μm of filter bag aperture with 0.1 g of sample weight and 60 min of extraction time.The rubber content of ‘Huazhong 6’ was 4.49% by filter bag method,higher than 3.64% by traditional Soxhlet's extraction.The rubber content in leaves from different clones and the same clone planted in different sites varied significantly (F=92.689, P=0.001).The highest rubber content of 7.00% was found in leaves of ‘Huazhong 11’ collected from Zhengzhou,while the lowest of 4.28% was from ‘Huazhong 12’ growing in Lingbao.Raw materials for Eucommia rubber industrialization were strongly recommended to be marked by Eucommia clone’s name,its place of origin and its age,which would be conducive for manufacturing enterprise to reasonably assess the expected rubber yield and production cost.

Key words: Eucommia ulmoides, Eucommia rubber content, extraction rate, weight lost method, filter bag technology

中图分类号: 

S792.99