天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (增刊2): 273-277.doi: 10.16333/j.10016880.2016.S.020

• 研究简报 • 上一篇    下一篇

紫外分光光度法测定青海产五种风毛菊属植物中的总黄酮

于瑞雪1,梅丽娟2,陶燕铎2,于瑞涛2*   

  1. 1平顶山学院,平顶山 467000;2中国科学院藏药研究重点实验室 中国科学院西北高原生物研究所,西宁 810001
  • 出版日期:2016-12-31 发布日期:2017-03-03

Determination of Total Flavonoids in Five Species of Saussurea DC from Qinghai Province by Ultraviolet Spectrophotometry

YU Ruixue1,MEI Lijuan2,TAO Yanduo2,YU Ruitao2*   

  1. 1Pingdingshan University,Pingdingshan 467000,China;2Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China
  • Online:2016-12-31 Published:2017-03-03

摘要: 为了分析青海产风毛菊属植物中总黄酮,本文采用三氯化铝比色法测定了五种风毛菊属植物中总黄酮的含量,该方法线性关系良好(r2=0.9986),平均回收率为99.10%,精密度和重复性试验RSD分别为1.15%和1.03%,表明本试验的重复性较好。结果显示五种风毛菊属植物水母雪莲、美丽风毛菊、重齿风毛菊、星状风毛菊、瑞苓草中总黄酮含量由高到低依次为美丽风毛菊9.53 mg/g、瑞苓草9.45 mg/g、星状风毛菊9.00 mg/g、重齿风毛菊8.95 mg/g、水母雪莲3.29 mg/g,RSD分别为1.18%、0.52%、0.70%、2.15%、0.60%。本实验结果可以为风毛菊属药用植物资源的深入研究和开发利用提供一定的理论依据。

关键词: 紫外分光光度法, 水母雪莲, 凤毛菊属, 总黄酮, 三氯化铝比色法

Abstract: To analyze the content of total flavonoids from five species of Saussurea DC from Qinghai province,AlCl3 colorimetry was applied to determine the content of five species of Saussurea DC,the method has good linear relationship(r2=0.9986),the average of recovery was 99.10%,the RSD of precision and repeatability test were 1.15% and 1.03%,respectively.The results showed that the content of total flavonoids of five species of Saussurea DC was 9.53mg/g of S.medusa,9.45 mg/g of S.nigrescens,9.00 mg/g of S.stella,8.95 mg/g of S.katochaete,3.29 mg/g of S.medusa from high to low order and the RSD was 1.18%,0.52%,0.70%,2.15% and 0.60%,respectively.The results will provide a theoretical basis for the further studies of the medicinal herbs of Saussurea DC species.

Key words: ultraviolet spectrophotometry, Saussurea medusa Maxim, Saussurea DC, total flavonoids, AlCl3 colorimetry

中图分类号: 

R284