天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (8): 1339-1348.doi: 10.16333/j.1001-6880.2017.8.014

• 研究简报 • 上一篇    下一篇

鄂尔多斯高原钝顶节旋藻SOD特性研究

包玉兰1,2,敖恩宝力格1*,乔辰2,巩东辉3   

  1. 1 内蒙古师范大学,呼和浩特 010022;2鄂托克旗螺旋藻工程技术研究中心,鄂尔多斯 016100;3内蒙古科技大学,包头 014010
  • 出版日期:2017-08-28 发布日期:2017-08-28

Characteristics of SOD in Arthrospira platensis from Erdos Plateau

BAO Yu-lan1,2,Aoenbaolige1*,QIAO Chen2,GONG Dong-hui3   

  1. 1 Inner Mongolia Normol University,Hohhot 010022,China;2 Etuoke Engineering Research Center for Spirulina, Ordos 016100,China;3 Inner Mongolia University of Science and Tecnology,Baotou 014010,China
  • Online:2017-08-28 Published:2017-08-28

摘要: 采用邻苯三酚自氧化法确定了鄂尔多斯高原钝顶节旋藻SOD特性。该SOD在紫外光区320 nm处有最大吸收值;在Tris-HCl缓冲溶液中最适pH值为8.2,最适温度为25 ℃;pH值稳定性范围为6~10;高于40 ℃的条件下保温20 min酶活性开始下降,高于35 ℃条件下保温60 min酶活性开始下降;室温下存放2 d后活性开始下降,7 d时活性基本丧失;室温下避光保存9 d时SOD活性完全丧失。

关键词: 钝顶节旋藻, 超氧化物岐化酶, 特性

Abstract: In this study,the pyrogallol auto-oxidation method was used to investigate the characteristics of superoxide dismutase (SOD) of Arthrospira platensis from the Erdos Plateau.The maximum absorption wavelength for SOD was 320 nm in the ultraviolet range.The optimal pH in a Tris-HCl buffer and the optimal temperature for SOD were 8.2 and 25 °C,respectively.The SOD activity was higher in a pH range of 6-10.Superoxide dismutase activity decreased at temperatures higher than 40 °C for more than 20 minutes and higher than 35 °C for more than 60 minutes.Similarly,SOD activity decreased at room temperature in light after a period of more than 2 days,almost not at all after seven days at room temperature in light,and exhibited absolutely no change at room temperature without light after nine days.

Key words: Arthrospira platensis, superoxide dismutase, characteristics