天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 35-39.doi: 10.16333/j.1001-6880.2019.S.007

• 研究论文 • 上一篇    下一篇

超声处理对生姜多糖理化特性和抗氧化活性的影响

贾凤霞,吴振,李孝彬,杨勇*   

  1. 重庆市中药研究院 中药健康学重庆市重点实验室,重庆400065
  • 出版日期:2019-08-06 发布日期:2019-08-06
  • 基金资助:

    重庆市重点产业共性关键技术创新专项(csct2017zdcy-zdyfx0013);重庆市集成示范计划(cstc2015 jcsf-kjfp80014)

Effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of ginger polysaccharides

JIA Feng-xia,WU Zhen,LI Xiao-bin,YANG Yong*   

  1. Chongqing Academy of Chinese Materia Medica,Chongqing Key Laboratory of Chinese medicine & Health Science,Chongqing 400065,China
  • Online:2019-08-06 Published:2019-08-06

摘要: 本文对比分析了热水提取生姜多糖(HWGPs)和超声提取生姜多糖(UEGPs)对生姜多糖(GPs)理化特性和抗氧化活性的影响。通过傅立叶变换中红外光谱、扫描电子显微镜、热重分析揭示超声处理对GPs结构特性的影响;采用清除DPPH·、OH·和O2能力评价超声处理对GPs抗氧化活性的影响。结果表明:超声处理并未改变生姜多糖的特征基团,但其特性和热力学特性均有变化,且其颗粒结构变小并易降解;UEGPs清除DPPH·和OH·的半抑制浓度(IC50)分别为2.72、1.94 mg/mL;HWGPs清除DPPH·和OH·的IC50要高于UEGPs,但清除O2的能力要强于UEGPs。GPs的抗氧化活性差异与超声处理导致多糖的聚集行为和热力学特性改变有关,为植物多糖的构效关系研究提供参考。

关键词: 生姜多糖, 超声处理, 理化特性, 抗氧化活性

Abstract: In this paper,ginger polysaccharides (GPs) were extracted by hot water method (HWGPs) and ultrasonic method (UEGPs).A comparative analysis of physicochemical properties and antioxidant activities were researched.The physicochemical properties of GPs were researched by FTIR,SEM and TG.The scavenging effects of GPs on DPPH free radical (DPPH·),hydroxyl free radical (OH·) and superoxide free radical (O2) were used to evaluate the antioxidant activities of GPs.The results showed that the ultrasonic treatment did not change the characteristic group of GPs,but the conformation property and the thermodynamic properties were changed,the particle structures become smaller and easily degradable.The half-inhibitory concentration (IC50) of UEGPs based on scavenging activities against DPPH· and OH· were 2.72 and 1.94 mg/mL respectively.The IC50 of HWGPs against DPPH· and OH· was higher than that of UEGPs,but the scavenging effect on O2 was stronger than that of UEGPs.The difference of antioxidant activities of GPs are relevant to change of conformation property thermodynamic properties caused by ultrasonic treatment.We provided a reference for the study on the structure-activity relationship of plant polysaccharides.

Key words: ginger polysaccharides, ultrasonic treatment, physicochemical properties, antioxidant activities

中图分类号: 

R282.4 R94