关键词: 七味净肝灵, 酒精性肝损伤, 氧化应激, 炎症反应, 白细胞分化抗原14, 核转录因子-&kappa, B

Abstract: To explore the protective effect and mechanism of Qi Wei Jing Gan Ling (QWJGL) on alcoholic liver injury in rats.60 rats were randomly divided into normal group,model group,silymarin group (0.18 g/kg),QWJGL high,medium and low dose group (8,4,2 g/kg),with 10 rats in each group.These groups were given by gavage once a day with 56%vol alcohol at a dose of 10 mL/kg per day,except normal group with distilled water.After 2 h,each drug group were given by gavage for 30 days with QWJGL,normal group and model group to give equal volume distilled water by gavage.After the last intragastric administration fasting for solids and liquids 24 h,reagent kits of ALT,AST,SOD,MDA and GSH-Px were employed for biochemical detection of serum,which isolated from blood sampled by eyeball extirpating.Taking out the liver,spleen and pancreas,and calculate LI,SI and TI.Serum IL-1β,IL-6 and TNF-α were detected by ELISA.CD14 and NF-κB protein levels in rat liver tissues were detected by Western blot.The histopathological changes of liver were observed by HE staining.The results showed that QWJGL reduced the LI of rats with alcoholic liver injury and improved SI and TI (P<0.05).The activity of ALT,AST and the content of MDA were reduced (P<0.05),the activity of SOD and GSH-Px was increased (P<0.05),and the level of TNF-α,IL-6 and IL-1β were reduced(P<0.05).And QWJGL could also inhibit the expression of NF-κB protein and CD14 receptor in liver tissue (P<0.05).It can be seen that the QWJGL has a significant protective effect on alcoholic liver injury,and its protective effect may be related to the inhibition of oxidative stress,inflammatory response,CD14 and NF-κB expressions.

Key words: Qi Wei Jing Gan Ling (QWJGL), alcoholic liver injury, oxidative stress, inflammatory response, cluster of differentiation antigen 14, nuclear factor-κB