天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (3): 387-394.doi: 10.16333/j.1001-6880.2019.3.003

• 研究论文 • 上一篇    下一篇

显齿蛇葡萄抗氧化活性与主要成分相关性研究

张命龙1,2，彭密军1,2*，杨秋玲2，王志宏1，王翔1   

  1. 1广东省测试分析研究所 广东省分析测试技术公共实验室，广州 510070；2广东中测食品化妆品安全评价中心有限公司，中山 528437
  • 出版日期:2019-04-02 发布日期:2019-04-02
  • 基金资助:

    广东省科学院发展专题(2019GDASYL-0502003);国家自然科学基金(31660181);湖南省教育厅重点项目(16A175)

Antioxidant properties and correlation with major chemical components in Ampelopsis grossedentata

ZHANG Ming-long1,2,PENG Mi-jun1,2*,YANG Qiu-ling2,WANG Zhi-hong1,WANG Xiang1   

  1. 1Guangdong Provincial Public Laboratory of Analysis and Testing Technology,Guangdong Institute of Analysis,Guangzhou 510070,China; 2GDZC food and cosmetics safety testing center Co.,Ltd.,Zhongshan 528437,China
  • Online:2019-04-02 Published:2019-04-02

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摘要： 探究不同显齿蛇葡萄提取物抗氧化活性及其与多酚、黄酮、二氢杨梅素的相关性。实验分别用水和乙醇溶液提取制备四种显齿蛇葡萄提取物，并测定总多酚、总黄酮、二氢杨梅素含量。利用·OH、O2-·、DPPH自由基清除实验以及还原力实验共四种体系，评价各提取物抗氧化活性；使用SPSS进行活性与成分的Pearson相关分析，并建立回归方程模型。结果显示，四种提取物均具有显著抗氧化活性，但差异明显，综合以二氢杨梅素提取物最强，醇提物居中，水提物最弱。Pearson相关分析显示不同提取物的抗氧化活性与总多酚、总黄酮、二氢杨梅素含量呈显著正相关（P<0.01），表明总多酚、总黄酮、二氢杨梅素均是显齿蛇葡萄中主要抗氧化功效因子，但不同抗氧化体系中三种成分的影响程度表现各异。

关键词: 显齿蛇葡萄, 多酚, 黄酮, 二氢杨梅素, 抗氧化, 相关性

Abstract: To explore the antioxidant properties of different extracts from Ampelopsis grossedentata and their correlation with polyphenols,flavonoids and dihydromyricetin.Four kinds of A.grossedentata extracts were prepared with water and ethanol.The content of total polyphenols,total flavonoids and dihydromyricetin in theseextracts was determined respectively.Antioxidant properties of A.grossedentata extracts were evaluated by four experiments,including scavenging activity of Superoxide radical (O2-·),hydroxyl radicals (·OH),DPPH radical and reducing power test.Pearson correlation analysis between properties and components was conducted with SPSS software.Meanwhile regression equation model was established.The results showed that antioxidant properties were dramatically different for four extracts,dihydromyricetin extract top,ethanol extracts mediate,and water extract bottom.Total polyphenols,flavonoids,dihydromyricetin content and antioxidant properties were significantly corelative (P < 0.01),which showed that polyphenols,flavonoids,dihydromyricetin were dominant antioxidant factors.However,the influence degree of these three components was discrepant in different antioxidant systems.

Key words: Ampelopsis grossedentata, polyphenols, flavonoids, dihydromyricetin, antioxidant, correlation

中图分类号: 

R284.1 R961

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