天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (3): 380-386.doi: 10.16333/j.1001-6880.2019.3.002

所属专题： No.8

• 研究论文 • 上一篇    下一篇

黄芩茎叶和藤茶抑菌作用及其联合应用研究

张琳1，宿晓蓉1，徐园1，许利嘉2，朱振华1，曾建国3，赵明1*，段金廒1*   

  1. 1南京中医药大学 江苏省中药资源产业化过程协同创新中心 中药资源产业化与方剂创新药物国家地方联合工程研究中心 国家中医药管理局中药资源循环利用重点研究室，南京 210023；2中国医学科学院北京协和医学院药用植物研究所，北京 100193；3湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程技术研究中心，长沙 410128
  • 出版日期:2019-04-02 发布日期:2019-04-02
  • 基金资助:

    国家重点研发计划（2017YFD0501500）；江苏省中药资源产业化过程协同创新中心开放课题（0120902002006-27）；江苏省研究生科研创新计划（KYCX18_1619）；国家现代农业产业技术体系建设专项（CARS-21）

Combination of stem and leave of Scutellaria baicalensis and Ampelopsis grossedentata increases their antibacterial efficacy

ZHANG Lin1,SU Xiao-rong1,XU-Yuan1,XU Li-jia2,ZHU Zhen-hua1,ZENG Jian-guo3,ZHAO Ming1*,DUAN Jin-ao1*   

  1. 1Jiangsu Collaborative Innovation Center of Chinese Medicinal Ｒesources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Ｒesources Industrialization and Formulae Innovative Medicine, Key Laboratory of Chinese Medicinal Resources Recycling Utilization of State
    Administration of Traditional Chinese Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China; 2Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100093,China; 3National and Provincial Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China
  • Online:2019-04-02 Published:2019-04-02

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摘要： 为探讨黄芩茎叶、藤茶及两者联合应用对金黄色葡萄球菌和痢疾杆菌的抑制作用，研究采用微量肉汤稀释法分别测定黄芩茎叶和藤茶对金黄色葡萄球菌和痢疾杆菌的最小抑菌浓度（Minimal inhibit concentration,MIC）及时间-杀菌曲线；采用棋盘式微量稀释法测定两者联合使用后的FIC指数。结果显示，黄芩茎叶和藤茶对金黄色葡萄球菌的MIC分别为1.0.25 mg/mL，对痢疾杆菌的MIC分别为4.0.50 mg/mL。两者联合使用，对金黄色葡萄球菌和痢疾杆菌的FIC指数分别为0.75、0.625，表现出相加的作用效果。研究提示基于联合使用，可进一步提高黄芩茎叶的抑菌效力，为实现黄芩茎叶的资源化利用提供科学依据。

关键词: 黄芩茎叶, 藤茶, 抑菌, 资源化利用

Abstract: To study the antibacterial effect of combination of the stem and leave of Scutellaria baicalensis (SLSB) and Ampelopsis grossedentata (AG) aginst Staphylococcus aureus and Shigella dysenteriae,the micro broth dilution method was used to detect their MIC values.The time-bactericidal curves of SLSB and AG against S.aureus and S.dysenteriae were established as well.A checkerboard method and the fractional inhibitory concentration index (FIC index) were used to evaluate the interaction between SLSB and AG.The results showed that the MIC values of SLSB and AG against S.aureus were 1 mg/mL and 0.25 mg/mL,while against S.dysenteriae were 4 mg/mL and 0.50 mg/mL,respectively.And the FIC indices of combined SLSB and AG,0.750 for S.aureus and 0.625 for S.dysenteriae,revealed their additive interaction against the tested bacterials.The present experimental results thus indicated the potential to increase the antibacterial efficacy of SLSB by rational combination strategy,providing a practical way for the rational utilization of SLSB resource.

Key words: stem and leave of Scutellaria baicalensis, Ampelopsis grossedentata, antibacterial, resource utilization

中图分类号: 

R285

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