天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (9): 1465-1471.doi: 10.16333/j.1001-6880.2022.9.002

• 研究论文 • 上一篇    下一篇

黄连内生真菌的分离鉴定及抑菌活性研究

杨   慧,李   钢,淮瑞平,杨丹妮,雷利杰,欧阳艳,熊莉丽*   

  1. 西南交通大学生命科学与工程学院,成都 610031
  • 出版日期:2022-09-28 发布日期:2022-10-08
  • 基金资助:
    中央高校基本科研业务费专项(2682020 ZT112);新疆维吾尔自治区高校科研计划(XJEDU2018I018)

Isolation,identification and antibacterial activity of endophytes of Coptis chinensis Franch.

YANG Hui,LI Gang,HUAI Rui-ping,YANG Dan-ni,LEI Li-jie,OUYANG-Yan,XIONG Li-li*   

  1. School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China
  • Online:2022-09-28 Published:2022-10-08

摘要:

为研究四川省峨眉地区黄连内生真菌多样性及菌株抑菌活性和机制,发掘黄连内生真菌资源,本研究从川黄连植株分离鉴定14株黄连内生真菌,分属于2纲,5目,7科,11属。根状茎、茎、叶和须根中的内生真菌定殖率分别是27.8%、56.7%、52.5%和39.6%。14株黄连内生真菌对金黄色葡萄球菌均有抑菌效果,Cop S01、Cop L01、Cop FR01的抑菌圈直径超过35 mm,纸片扩散法测得3株内生真菌发酵液对金黄色葡萄球菌均有效,Cop L01抑菌圈直径大于20 mm,抑菌效果最好,其发酵产物最低抑菌浓度(MIC)为10 mg/mL,最低杀菌浓度(MBC)为11 mg/mL。初步探究Cop L01抑菌机制发现该菌株发酵产物破坏金黄色葡萄球菌细胞膜,引起胞内核酸及蛋白外泄,造成金黄色葡萄球菌死亡。本研究表明川黄连内生真菌具有丰富的多样性,部分菌株及其发酵产物对金黄色葡萄球菌均表现出较好的抑菌效果,预示着黄连内生真菌Cop L01具有产生新型抗菌活性物质的潜力,为后续深入开发这一资源宝库提供了新的菌种和思路。

关键词: 黄连, 内生真菌, 抑菌活性

Abstract:

To study on the diversity of endophyte and the antibacterial activity and mechanism of the strains in Emei area of Sichuan Province,and explored the endophytes resources of Coptis chinensis Franch.,identified as 14 species by molecular biology and morphological microscopy,belonged to 2 classes,5 orders,7 families,11 genera.The colonization rates of endophytes in rhizomes,stems,leaves,and fibrous roots were 27.8%,56.7%,52.5%,and 39.6%.Fourteen endophytes from C. chinensis have an antibacterial effect on Staphylococcus aureus,and the diameter of inhibition zone of Cop S01,Cop L01,and Cop FR01 strains is more than 35 mm,the fermentation broth of the three strains of endophytes was found to be effective against S. aureus by diffusion method,and the diameter of the inhibition zone of strain Cop L01 was greater than 20 mm.The minimum inhibitory concentration (MIC) of the fermentation product of this strain was 10 mg/mL,its minimum bactericidal concentration (MBC) was 11 mg/mL.The inhibition mechanism of Cop L01 showed that the product damaged the cell membrane of S. aureus,caused the leakage of intracellular nucleic acid and protein,resulting in the death of S. aureus.This study showed that the endophytes of C. chinensis are rich in diversity,and some strains and their products showed good antibacterial effect on S. aureus.Cop L01 have potential to produce new antibacterial active substances.This provided new strains and ideas for later exploitation of endophyte.

Key words: Coptis chinensis Franch., endophyte, antibacterial activity

中图分类号:  Q93-331