天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (3): 420-426.doi: 10.16333/j.1001-6880.2023.3.008

• 研究简报 • 上一篇    下一篇

细果角茴香内生真菌X-396的分离鉴定及其发酵物脂溶性成分分析

谭   慧1,3，皮   立1，杨小龙1，安   婷1,3，郭旭栋2，刘艳萍2，文怀秀1*   

  1. 1中国科学院西北高原生物研究所 青海省藏药研究重点实验室，西宁 810008；2青海大学农林科学院，西宁 810016；3中国科学院大学，北京 100049
  • 出版日期:2023-03-28 发布日期:2023-03-28
  • 基金资助:
    青海省科技厅项目（2021-SF-SW1）；“青海省藏药研究重点实验室”（2022-ZJ-Y03）

Isolation and indentification of an endophytic fungus X-396 from Hypecoum leptocarpum Hook.f.et Thoms.and analysis on fat-soluble components of its fermentation

TAN Hui1,3,PI Li1,YANG Xiao-long1,AN Ting1,3,GUO Xu-dong2,LIU Yan-ping2,WEN Huai-xiu1*   

  1. 1Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China; 2Research Institute of Forestry,Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining 810016,China;3University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2023-03-28 Published:2023-03-28

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摘要：

以藏药细果角茴香叶为材料，采用组织分离法分离纯化出一株内生真菌X-396。 经形态学观察、ITS 测序与序列比对鉴定内生真菌X-396为间座壳属（Diaporthe sp.）真菌Diaporthe sp.YG-2015。进一步利用气相色谱-质谱（GC-MS）联用技术对该真菌发酵物脂溶性成分进行检测与分析，并通过CCK-8法测定其抗肿瘤活性。结果表明，发酵物脂溶性成分共鉴定出35个，其中含量较高的成分为麦角甾醇（31.83%）、β-谷甾醇（12.00%）和十六酸甲酯（4.22%）；体外抗肿瘤活性测试显示，发酵物脂溶性成分对人肿瘤细胞株A549、MCF-7和HepG2均具有不同程度的抑制作用，其中对HepG2抑制效果最好，IC50为22.65 μg/mL。本研究表明细果角茴香内生真菌X-396发酵物脂溶性成分中具有产生抗肿瘤活性化合物的潜力，具有进一步深入研究的价值。

关键词: 细果角茴香, 内生真菌, 发酵物, 脂溶性成分, GC-MS, 抗肿瘤 

Abstract:

An endophytic fungus X-396 was isolated from the leaves of Hypecoum leptocarpum Hook.f.et Thoms. through a tissue separating method.The endophytic fungus X-396 was identified as Diaporthe sp.YG-2015 according to the morphologic observation and ITS sequence analysis.Its fat-soluble components of fermentation were further studied with GC-MS,and antitumor activity was measured by CCK-8 assay.35 kinds of fat-soluble components were detected and identified.The findings showed that the main components of fat-soluble components in fermentation of X-396 were ergosterol (31.83%), β-stitosterol (12.00%) and methyl hexadecanoate (4.22%).Antitumor activity test in vitro showed that the fat-soluble components of fermentation had different degrees of inhibitory effects on human tumor cell lines A549,MCF-7 and HepG2,and HepG2 had the best inhibitory effect with IC50 of 22.65 μg/mL.The results indicate that the fat-soluble components of endophytic fungus X-396 fermentation have the potential to produce antitumor active compounds,and deserve to be deeply investigated.

Key words: Hypecoum leptocarpum Hook.f.et Thoms., endophytic fungus, fermentation, fat-soluble components, GC-MS, antitumor

中图分类号:  R284.1

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