天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (8): 1434-1438.doi: 10.16333/j.1001-6880.2019.8.019

• 开发研究 • 上一篇    下一篇

链霉菌KIB-H1556次级代谢产物抗植物真菌活性研究

董庭邦1,王磊2,桂潇1,苏灿2,李洁2,黄胜雄2*,王福生1*   

  1. 1大理大学药学与化学学院,大理 671000;2中国科学院昆明植物研究所 植物化学与西部植物资源持续利用国家重点实验室,昆明 650201
  • 出版日期:2019-09-10 发布日期:2019-09-10
  • 基金资助:

    国家自然科学基金(31860098)

Metabolites against plant pathogenic fungi from Streptomyces sp.KIB-H1556

DONG Ting-bang1,WANG Lei2,GUI Xiao1,SU Can2,LI Jie2,HUANG Sheng-xiong2*,WANG Fu-sheng1*   

  1. 1College of Pharmacy and Chemistry,Dali University,Dali 671000,China; 2State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China
  • Online:2019-09-10 Published:2019-09-10

摘要: 对曼陀罗(Datura stramonium L.)根际链霉菌Streptomyces sp.KIB-H1556的次级代谢产物进行研究,利用硅胶柱色谱、凝胶柱色谱和半制备HPLC等分离手段对其发酵产物进行分离纯化,采用MS和NMR等波谱学手段并结合文献数据鉴定了3个单体化合物的结构,分别为:Bafilomycin D (1)、Bafilomycin B1 (2)和Bafilomycin B2 (3)。初步抗植物病原真菌活性筛选发现化合物2和3具有广谱抗真菌活性,尤其对玉米病原真菌的抑制活性显著,可作为玉米病原真菌病害的潜在生物防治剂。

关键词: 链霉菌, 次级代谢产物, 大环内酯类, 抗真菌活性

Abstract: The secondary metabolites of Streptomyces sp.KIB-H1556 that an actinomycetes isolated from the rhizosphere soil of Datura stramonium L.were investigated.The extracts were purified by silica gel column,Sephadex LH-20 chromatography and semi-preparative HPLC.The structures of compounds were elucidated by NMR and mass spectrometry,and comparison with the reported data.Three compounds were identified as Bafilomycin D (1),Bafilomycin B1 (2) and Bafilomycin B2 (3).The antifungal assays demonstrated that compounds 2 and 3 possessed broad-spectrum antifungal activities against pathogenic fungi,especially pathogenic fungi related to corn.This result suggests that bafilomycins can been developed as potential antifungal agent for corn.

Key words: Streptomyces, secondary metabolites, macrolides, antifungal activity

中图分类号: 

R914.4 R284.2