关键词: 枸杞多糖, 分子量范围, 乙醇, 肝细胞损伤

Abstract:

To explore the protective effect of Lycium barbarum polysaccharides (LBPs) on ethanol-induced hepatocyte injury.Crude polysaccharides (LBPs0) and different molecular weight ranges LBPs (LBPs1-LBPs4) were extracted and separated from Lycium barbarumsamples,human normal hepatocytes (L-02 cells) were used to evaluate the protective activity of LBPs on ethanol-induced hepatocyte injury:Cell activity was detected by MTT assay,the release of ALT,AST and LDH in cell culture was detected by colorimetry,and intracellular reactive oxygen species (ROS) were detected by DCFH -DA assay.The results showed that LBPs had no inhibition or promotion effect on cell proliferation between 0-200 μg/mL,and could protect ethanol-induced hepatocyte injury by increasing cell activity,reducing the release of ALT,AST and LDH,and inhibiting intracellular ROS production.The activity of LBPs in different molecular weight ranges was different,LBPs3 was the strongest,followed by LBPs4 and LBPs2,and LBPs1 was the weakest.In conclusion,LBPs can protect ethanol-induced hepatocyte injury,and LBPs3 with a molecular weight range of 5-10 kDa has the highest activity,the results of this experiment can provide experimental basis and theoretical guidance for the application and development of LBPs to protect liver injury functional food and drug.

Key words: Lycium barbarum polysaccharides, molecular weight range, ethanol, hepatocyte injury