关键词: 层次分析-熵权法, 中心复合设计-响应面法, 酒蒸高乌头根, 总生物碱, 高乌甲素, 冉乌头碱

Abstract:

This study aims to explore the optimal processing parameters of the Aconitum sinomontanum root.The comprehensive score of total alkaloid,lappaconitine and ranaconitine content were used as the evaluation indexes.The total alkaloid content was determined by ultraviolet spectrophotometry,and the lappaconitine and ranaconitine contents were measured by ultra-high performance liquid chromatography.The single factor experiment were conducted to determine the effects of the amount of added Huangjiu,moistening time,and steaming time.The weight of the three components were determined by analytic hierarchy process combined with entropy weight method.The optimal processing parameters of Huangjiu-steamed A.sinomontanum root were determined by the comprehensive score combined with central composite design-response surface method.The results showed that the optimal processing parameters for Huangjiu-steamed A.sinomontanum root was as follows:15% of the weight of A.sinomontanum root as the amount of added Huangjiu,a moistening time of 2 h,a steaming time of 5 h,a steaming temperature of 127 ℃,and a steaming pressure of 0.15 MPa.This study broadens the processing methods of A.sinomontanum root.The established processing evaluation system for the processing technology is accurate and reliable,and can provide references for related research and clinical safety applications of A.sinomontanum root.

Key words: analytic hierachy process-entropy weight method, central composite design-response surface method, Huangjiu-steamed Aconitum sinomontanum Nakai root, total alkaloids, lappaconitine, ranaconitine