关键词: 天然有机物, 铁皮石斛, 原球茎, 多糖, 总生物碱

Abstract: The aim of this paper was to provide a new way of the production of BPCs in Dendrobium officinale.Taking MS as minimal medium,adding nature organics,the effects on the growth of protocorm-like bodies(PLBs) from Dendrobium officinale and the major medicinal components including polysaccharide and total alkaloid were discussed in different incubation time.The results showed that the best concentration of potato juice was 250 g/L,the dry-weight percentage and polysaccharide contents both reached a maximum on the 30 days,which were 8.944% and 17.921% respectively,the highest proliferation rate was 1 028.94% on the 50 days,while the total alkaloid contents were accumulated on the 40 days,the maximum was 0.031 61%.The proliferation rate,polysaccharide and total alkaloid contents were significantly higher than the contrast with adding hormones.The variation of all indexes with adding tomato juice to the medium was similar to the potato juice,but the growth of PLBs was weak and the colour was yellow,the proliferative ability was poorer than adding potato juice.Taken together,the medium of MS+250 g/L potato juice+30 g/L sucrose+7 g/L agar was beneficial for the growth of PLBs from Dendrobium officinale and accumulating active ingredients,which could be considered as a polysaccharide and alkaloid production source,and it had very important practical significances to produce major medical ingredients of PLBs from Dendrobium officinale.

Key words: nature organics, Dedrobium officinale, protocorm-like bodies, polysaccharide, total alkaloid