天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (5): 816-827.doi: 10.16333/j.1001-6880.2025.5.003 cstr: 32307.14.1001-6880.2025.5.003

• 研究论文 • 上一篇    下一篇

基于UPLC-QE Orbitrap MS/MS技术分析老虎芋水提物入血成分

罗慧敏，朱  华*，许立拔，王冰冰，李梅林，陆  璐，张  淼*，陈  龙   

  1. 广西中医药大学 广西壮瑶药重点实验室 壮瑶药材开发及产业化工程研究中心 壮瑶药协同创新中心 广西壮族自治区民族药资源与应用工程研究中心，南宁 530200
  • 出版日期:2025-05-28 发布日期:2025-05-26
  • 基金资助:
    广西科技基地和人才专项(桂科AD21238031)；国家自然科学基金(82060695)；壮瑶药协同创新中心项目(ZYYDC202203)；广西重点研发计划(桂科AB21196016)

Analysis of blood-absorbed components of Alocasiae Cucullatae Rhizoma aqueous extract based on UPLC-QE Orbitrap MS/MS

LUO Hui-min, ZHU Hua *, XU Li-ba, WANG Bing-bing, LI Mei-lin, LU Lu, ZHANG Miao*, CHEN Long   

  1. Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Engineering Research Center for Development and Industrialization of Zhuang and Yao Medicinal Materials, Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Research Center of Engineering, Guangxi University of Chinese Medicine, Nanning 530200, China
  • Online:2025-05-28 Published:2025-05-26

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摘要：

本研究采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术（UPLC-QE Orbitrap MS/MS）鉴定老虎芋水提物（Alocasiae Cucullatae Rhizoma aqueous extract，ACRAE）及大鼠血清中的化学成分。色谱分离以水（含0.1%甲酸）-乙腈为流动相进行梯度洗脱，流速0.35 mL/min，进样量5 μL。质谱检测采用加热电喷雾电离（HESI）离子源，分别在正、负离子模式下采集质谱信号，扫描范围设置为m/z 100~1 200。通过比对ACRAE、空白血清和含药血清的图谱差异，根据保留时间、相对分子质量，以及一级、二级离子碎片，解析ACRAE经大鼠灌胃给药后血清中的原型成分和代谢产物。ACRAE鉴定得到135个化学成分。在血清中检测到68个入血成分，其中23个为原型成分，45个为代谢产物。通过UPLC-QE Orbitrap MS/MS技术快速分析ACRAE的入血成分，为进一步阐明老虎芋的药效物质基础及作用机制提供了参考。

关键词: 老虎芋, UPLC-QE Orbitrap MS/MS, 入血成分, 原型成分, 代谢产物

Abstract:

This study utilized ultra-high-performance liquid chromatography coupled with quadrupole-electrostatic field orbitrap high-resolution tandem mass spectrometry (UPLC-QE Orbitrap MS/MS) to identify the chemical components in Alocasiae Cucullatae Rhizoma aqueous extract (ACRAE) and rat serum. Chromatographic separation was performed using a gradient elution with a mobile phase of water (containing 0.1% formic acid) and acetonitrile at a flow rate of 0.35 mL/min, with an injection volume of 5 μL. Mass spectrometric analysis was carried out with a heated electrospray ionization (HESI) source, and data were acquired in both positive and negative ion modes over a scanning range of m/z 100–1 200. By comparing the chromatographic profiles of ACRAE, blank serum, and drug-containing serum, prototype components and metabolites in the serum after oral administration were identified based on retention times, molecular weights, and both primary and secondary ion fragments. A total of 135 chemical components were characterized in the ACRAE. In serum, 68 blood-absorbed components were detected, including 23 prototype components and 45 metabolites. The rapid profiling of blood-absorbed components in ACRAE via UPLC-QE Orbitrap MS/MS provides critical insights for further exploration of its pharmacodynamic material basis and mechanisms of action.

Key words: Alocasiae Cucullatae Rhizoma, UPLC-QE Orbitrap MS/MS, blood-absorbed components, prototype components, metabolites

中图分类号:  R917 

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