天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (6): 1051-1054.doi: 10.16333/j.1001-6880.2025.6.007 cstr: 32307.14.1001-6880.2025.6.007

• 研究简报 • 上一篇    下一篇

三七内生真菌Nigrospora sp.中一个新的聚酮化合物

陈浩阳1，张继宇2，徐孟飞2，付硕硕2，徐会有2*，倪 林2*   

  1. 1中国药科大学工学院，南京 211100；2福建农林大学植物保护学院，福州 350000
  • 出版日期:2025-06-25 发布日期:2025-06-25
  • 基金资助:
    福建特色植物资源开发与利用(KH210298A)；药用植物产业化工程技术联合实验室共建项目(KH210026A)

A new polyketide produced by Nigrospora sp.,an endophytic fungus from Panax notoginseng

CHEN Hao-yang1,ZHANG Ji-yu2,XU Meng-fei2,FU Shuo-shuo2,XU Hui-you2*,NI Lin2*   

  1. 1School of Engineering,China Pharmaceutical University,Nanjing 211100,China;2College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2025-06-25 Published:2025-06-25

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摘要：

研究三七内生真菌Nigrospora sp.的化学成分及其生物活性。应用硅胶柱色谱、高效液相色谱等多种色谱分离技术，从该菌株土豆液体发酵产物的乙酸乙酯提取物中分离得到3个聚酮类化合物，根据NMR、HR-ESI-MS、ECD等方法确定其结构，分别鉴定为(R)-2-methyl-4-(2-oxobutyl) cyclopent-2-en-1-one （1）、multiplolide A（2）、8-O-acetylmultiplolide（3）。其中，化合物1是一个新的环戊烯酮类衍生物，化合物2和3是十元大环内酯类衍生物，均为首次从Nigrospora sp.分离得到。抗菌活性实验结果表明，化合物1对大肠杆菌（Escherichia coli）具有微弱的抗菌活性，最小抑菌浓度为64 µg/mL。

关键词: 内生真菌, Nigrospora sp., 聚酮, 抗菌活性

Abstract:

To study the chemical components and biological activity of the endophytic fungus Nigrospora sp. from Panax notoginseng, various chromatographic separation techniques, including silica gel column chromatography and high-performance liquid chromatography, were employed. Three polyketide compounds were isolated from the ethyl acetate extract of the potato liquid fermentation products of this strain. Their structures were determined using methods such as NMR, HR-ESI-MS, and ECD, and were identified as (R)-2-methyl-4-(2-oxobutyl)cyclopent-2-en-1-one (1), multiplolide A (2), and 8-O-acetylmultiplolide (3). Among them, compound 1 was a new cyclopentenone derivative, while compounds 2 and 3 were derivatives of ten-membered macrolides, all of which were isolated from Nigrospora sp. for the first time. Antibacterial activity results indicated that compound 1 exhibited weak antibacterial activity against Escherichia coli, with a minimum inhibitory concentration of 64 µg/mL.

Key words: endophytic fungus, Nigrospora sp., polyketide, antibacterial activity

中图分类号:  R284.2

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