天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (6): 1070-1076.doi: 10.16333/j.1001-6880.2025.6.010 cstr: 32307.14.1001-6880.2025.6.010

• 研究简报 • 上一篇    下一篇

甘肃棘豆的化学成分及抗疟活性研究

雷 丹1,2，苟 亮1,2，孙 河1,2，卯 锦1,2，陈启银1,2，肖朝江1,2，姜 北1,2*，张永增1,2*   

  1. 1云南省滇西抗病原植物资源筛选研究重点实验室；2大理大学药学院&药物研究所，大理 671000
  • 出版日期:2025-06-25 发布日期:2025-06-25
  • 基金资助:
    云南省自然科学基金(202401CF070015)；云南省滇西抗病原植物资源筛选研究重点实验室项目(202305 AG340015)

Chemical constituents and antimalarial activity of Oxytropis kansuensis

LEI Dan1,2,GOU Liang1,2,SUN He1,2,MAO Jin1,2,CHEN Qi-yin1,2,XIAO Chao-jiang1,2,JIANG Bei1,2*,ZHANG Yong-zeng1,2*   

  1. 1Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from Western Yunnan;2College of Pharmacy & Institute of Materia Medica,Dali University,Dali 671000,China
  • Online:2025-06-25 Published:2025-06-25

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摘要：

研究高寒地区甘肃棘豆Oxytropis kansuensis Bunge干燥全株的化学成分和抗疟活性。分离和纯化过程采用硅胶、D101大孔树脂、Sephadex LH-20等多种柱色谱方法；根据理化性质及波谱数据鉴定化合物的结构；采用SYBR Green I法，对分离得到的化合物进行抗疟活性筛选。从甘肃棘豆中分离鉴定了10个化合物，分别鉴定为β-谷甾醇亚麻酸酯（1）、羽扇豆酮（2）、β-谷甾醇（3）、9,12,15-十八烷三烯酸甲酯（4）、芹菜素（5）、木犀草素（6）、1-O-反式对香豆酰甘油（7）、1-O-p-coumaroyl-3-O-feruloylglycerol（8）、胡萝卜苷（9）、芹菜素-7-O-β-D-吡喃葡萄糖苷（10）。化合物1作为天然产物首次报道，本文还首次报道了其1H NMR、13C NMR数据。此外，化合物2、4 ~ 8、10为首次从甘肃棘豆中分离得到，化合物1、4 ~ 6、8、10对恶性疟原虫3D7株具有一定抗疟活性。

关键词: 甘肃棘豆, 化学成分, 抗疟活性, SYBR Green I法, β-谷甾醇亚麻酸酯

Abstract:

The chemical constituents and antimalarial activity of Oxytropis kansuensis Bunge from the alpine region were studied. The compounds were separated and purified by a series of chromatographic techniques, including silica gel chromatography, D101 macroporous resin, and Sephadex LH-20 column chromatography. The structures of the compounds were identified on the basis of physicochemical properties and spectral data, and their antimalarial activities were screened using the SYBR Green I method. Ten compounds were isolated and identified from O. kansuensis, namely β-sitosteryl linolenate (1), lupenone (2), β-sitosterol (3), 9,12,15-octadecatrienoic acid, methyl ester (4), apigenin (5), luteolin (6), 1-O-trans-p-coumaroylglycerol (7), 1-O-p-coumaroyl-3-O-feruloylglycerol (8), daucosterol (9), and andcosmosiin (10). Compound 1 was reported for the first time as a natural product, and its NMR data was also reported for the first time. Compounds 2, 4-8 and 10 were isolated from O. kansuensis for the first time, and compounds 1, 4-6, 8 and 10 showed antimalarial activity against Plasmodium falciparum strain 3D7.

Key words: Oxytropis kansuensis, chemical composition; antimalarial activity, SYBR Green I method, β-sitosteryl linolenate

中图分类号:  R284.2

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